Recombinant humaan 17 bèta-HSD14/HSD17B14-eiwit

HSD17B14, also known as 17-beta-hydroxysteroid dehydrogenase 14, belongs to the 17-beta-HSD family of proteins, which regulate the availability of steroids within various tissues rough out the body. It exists as a homotetramer that localizes to the cytoplasm and is highly expressed in brain, placenta, liver and kidney. Recombinant human HSD17B14 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Hydroxysteroid 17-beta dehydrogenase 14, 17-beta-hydroxysteroid dehydrogenase 14, 17-beta-hydroxysteroid dehydrogenase DHRS10, Dehydrogenase/reductase SDR family member 10, DHRS10, Retinal short-chain dehydrogenase/reductase retSDR3, SDR3, Short chain dehydrogenase/reductase family 47C member 1, SDR47C1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

32.4 kDa (306aa) confirmed by MALDI-TOF

Short chain dehydrogenase/reductase family 47C member 1, SDR47C1, SDR3, retSDR3, Retinal short-chain dehydrogenase/reductase retSDR3, Hydroxysteroid 17-beta dehydrogenase 14, HSD17B14, DHRS10, Dehydrogenase/reductase SDR family member 10, 17-beta-hydroxysteroid dehydrogenase DHRS10, 17-beta-hydroxysteroid dehydrogenase 14, 17 b-HSD14, ATGP0798-10 µg, ATGP0798-20 µg, ATGP0798-50 µg, ATGP0798-100 µg, ATGP0798-250 µg, ATGP0798-500 µg, ATGP0798-1 mg, ATGP0798-010, ATGP0798-020, ATGP0798-050, ATGP0798-100, ATGP0798-250, ATGP0798-500, ATGP0798-01M

Grimwood J., et al. (2004), Nature., 428: 529-535.; ; Lukacik P., et al. (2007) . Biochem. J., 402: 419-427

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Redox Proteins

NP_057330

Q9BPX1

Human

MATG TRYAGKVVVV TGGGRGIGAG IVRAFVNSGA RVVICDKDES GGRALEQELP GAVFILCDVT QEDDVKTLVS ETIRRFGRLD CVVNNAGHHP PPQRPEETSA QGFRQLLELN LLGTYTLTKL ALPYLRKSQG NVINISSLVG AIGQAQAVPY VATKGAVTAM TKALALDESP YGVRVNCISP GNIWTPLWEE LAALMPDPRA TIREGMLAQP LGRMGQPAEV GAAAVFLASE ANFCTGIELL VTGGAELGYG CKASRSTPVD APDIPS