Recombinant humaan 17 bèta-HSD1/HSD17B1-eiwit

Estradiol 17-beta-dehydrogenase 1, also known as HSD17B1, belongs to the short-chain dehydrogenases/reductases (SDR) family. This protein favors the reduction of estrogens and androgens. Recombinant human HSD17B1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Hydroxysteroid 17-beta dehydrogenase 1 (approved name), 17-beta-hydroxysteroid dehydrogenase type 1, 17-beta-HSD 1, 20 alpha-hydroxysteroid dehydrogenase, 20-alpha-HSD, E2DH, Estradiol 17-beta-dehydrogenase 1, Placental 17-beta-hydroxysteroid dehydrogenase, Short chain dehydrogenase/reductase family 28C member 1, HSD17B1, E17KSR, EDH17B1, EDH17B2, EDHB17, SDR28C1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

37.5 kDa (352aa) confirmed by MALDI-TOF

Short chain dehydrogenase/reductase family 28C member 1, SDR28C1, Placental 17-beta-hydroxysteroid dehydrogenase, Placental 17 beta hydroxysteroid dehydrogenase, Hydroxysteroid 17-beta dehydrogenase 1 (approved name), Hydroxysteroid 17-beta dehydrogenase 1, Hydroxysteroid 17 beta dehydrogenase 1, HSD17B1, Estradiol 17-beta-dehydrogenase 1, Estradiol 17 beta dehydrogenase 1, EDHB17, EDH17B2, EDH17B1, E2DH, E17KSR, 20-alpha-HSD, 20 alpha-hydroxysteroid dehydrogenase, 20 alpha hydroxysteroid dehydrogenase, 20 alpha HSD, 17-beta-hydroxysteroid dehydrogenase type 1, 17-beta-HSD 1, 17 beta hydroxysteroid dehydrogenase type 1, 17 beta HSD 1, ATGP2101-10 µg, ATGP2101-20 µg, ATGP2101-50 µg, ATGP2101-100 µg, ATGP2101-250 µg, ATGP2101-500 µg, ATGP2101-1 mg, ATGP2101-010, ATGP2101-020, ATGP2101-050, ATGP2101-100, ATGP2101-250, ATGP2101-500, ATGP2101-01M

Qiu W., et al. (2002) FASEB J. 16:1829-1831; ; Gangloff A. (2003) FASEB J. 17:274-276

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_000404

P14061

Human

MARTVV LITGCSSGIG LHLAVRLASD PSQSFKVYAT LRDLKTQGRL WEAARALACP PGSLETLQLD VRDSKSVAAA RERVTEGRVD VLVCNAGLGL LGPLEALGED AVASVLDVNV VGTVRMLQAF LPDMKRRGSG RVLVTGSVGG LMGLPFNDVY CASKFALEGL CESLAVLLLP FGVHLSLIEC GPVHTAFMEK VLGSPEEVLD RTDIHTFHRF YQYLAHSKQV FREAAQNPEE VAEVFLTALR APKPTLRYFT TERFLPLLRM RLDDPSGSNY VTAMHREVFG DVPAKAEAGA EAGGGAGPGA EDEAGRGAVG DPELGDPPAA PQ