Recombinant humaan 17 bèta-HSD8/HSD17B8 eiwit

HSD17B8, also known as estradiol 17-beta-dehydrogenase 8, belongs to the short-chain dehydrogenases/reductases (SDR) family. In mice, the Ke6 protein is a 17-beta-hydroxysteroid dehydrogenase that can regulate the concentration of biologically active estrogens and androgens. It is preferentially an oxidative enzyme and inactivates estradiol, testosterone, and dihydrotestosterone. However, the enzyme has some reductive activity and can synthesize estradiol from estrone. It may play a role in biosynthesis of fatty acids in mitochondria. Recombinant human HSD17B8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Hydroxysteroid 17-beta dehydrogenase 8, (3R) -3-hydroxyacyl-CoA dehydrogenase, FABGL, HKE6, RING2, SDR30C1, 17-beta-hydroxysteroid dehydrogenase 8, 17-beta-HSD 8, HSD17B8, 3-ketoacyl-[acyl-carrier-protein, reductase alpha subunit, KAR alpha subunit, 3-oxoacyl-[acyl-carrier-protein, reductase, Estradiol 17-beta-dehydrogenase 8, Protein Ke6, Ke6, Short chain dehydrogenase/reductase family 30C member 1, Testosterone 17-beta-dehydrogenase 8, H2-KE6

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

29.1 kDa (281aa) confirmed by MALDI-TOF

HSD17B8, ATGP1001-10 µg, ATGP1001-20 µg, ATGP1001-50 µg, ATGP1001-100 µg, ATGP1001-250 µg, ATGP1001-500 µg, ATGP1001-1 mg, ATGP1001-10, ATGP1001-20, ATGP1001-50, ATGP1001-100, ATGP1001-250, ATGP1001-500, ATGP1001-1

Ando A., et al. (1996) Genomics. 35:600-602; ; Rotinen M., et al. (2009) Endocrinol 200:85-92

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 40% glycerol, 150 mM NaCl

Metabolism

NP_055049.1

Q92506

Human

MASQLQNRLR SALALVTGAG SGIGRAVSVR LAGEGATVAA CDLDRAAAQE TVRLLGGPGS KEGPPRGNHA AFQADVSEAR AARCLLEQVQ ACFSRPPSVV VSCAGITQDE FLLHMSEDDW DKVIAVNLKG TFLVTQAAAQ ALVSNGCRGS IINISSIVGK VGNVGQTNYA ASKAGVIGLT QTAARELGRH GIRCNSVLPG FIATPMTQKV PQKVVDKITE MIPMGHLGDP EDVADVVAFL ASEDSGYITG TSVEVTGGLF M