Recombinant humaan 4-1BB Ligand/TNFSF9 eiwit

4-1BB ligand is a type 2 transmembrane glycoprotein belonging to the TNF superfamily. 4-1BBL is expressed by activated B cells, macrophages, dendritic cells, activated T cells, neurons and astrocytes. The interaction of 4-1BB with 4-1BBL strongly regulates immunity and has been proposed to preferentially control T cell responses based on studies in various murine models of cancer, infectious disease and autoimmune disease. Recombinant human 4-1BB ligand, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Human TNFSF9 is coated at 2 µg/mL (100 ul/well) can bind Human TNFRSF9 (CAT# ATGP3303) in a Functional ELISA assay.

Tumor necrosis factor ligand superfamily member 9, 4-1BB ligand, 4-1BBL, receptor 4-1BB ligand, homolog of mouse 4-1BB-L, CD137 ligand, CD137L

E.coli

Human

His-Tag

SDS-PAGE, Bioactivity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Measured by its binding ability in a functional ELISA with Human TNFRSF9 (CAT# ATGP3303) .

23.8 kDa (222aa) confirmed by MALDI-TOF

41BB Ligand, TNFSF9, Tumor necrosis factor ligand superfamily member 9, TNFSF9, 4-1BBL, CD137L, 4-1BB ligand, 4 1BB L, 4 1BB ligand, 4 1BBL, Cd137l, Cd157l, homolog of mouse 4 1BB L, homolog of mouse 4 1BBL, ILA ligand (TNF related), Ly63l, receptor 4 1BB ligand, Tumor necrosis factor (ligand) superfamily member 9., ATGP0308-10 µg, ATGP0308-20 µg, ATGP0308-50 µg, ATGP0308-100 µg, ATGP0308-250 µg, ATGP0308-500 µg, ATGP0308-1 mg, ATGP0308-10, ATGP0308-20, ATGP0308-50, ATGP0308-100, ATGP0308-250, ATGP0308-500, ATGP0308-1

Lee SW., et al. (2008) . Nat Immunol. 9 (8) :917-26; ; Sharma RK., et al. (2009) . Cancer Res. 69 (10) :4319-26;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 100 mM NaCl, 20% glycerol

Hormones & Cytokines

NP_003802

P41273

Human

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSHMRE GPELSPDDPA GLLDLRQGMF AQLVAQNVLL IDGPLSWYSD PGLAGVSLTG GLSYKEDTKE LVVAKAGVYY VFFQLELRRV VAGEGSGSVS LALHLQPLRS AAGAAALALT VDLPPASSEA RNSAFGFQGR LLHLSAGQRL GVHLHTEARA RHAWQLTQGA TVLGLFRVTP EIPAGLPSPR SE