Recombinant humaan 4-1BB/CD137/TNFRSF9 eiwit

TNFRSF9, also known as tumor necrosis factor receptor superfamily member 9, is a member of the TNF-receptor superfamily. This receptor contributes to the clonal expansion, survival, and development of T cells. It can also induce proliferation in peripheral monocytes, enhance T cell apoptosis induced by TCR/CD3 triggered activation, and regulate CD28 co-stimulation to promote Th1 cell responses. The expression of this receptor is induced by lymphocyte activation. TRAF adaptor proteins have been shown to bind to this receptor and transduce the signals leading to activation of NF-kappaB. Recombinant human TNFRSF9, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Human TNFSF9 (CAT# ATGP0308) is coated at 2 µg/mL (100 ul/well) can bind Human TNFRSF9. The ED50 range ≤ 50 ng/mL.

Tumor necrosis factor receptor superfamily member 9, 4-1BB ligand receptor, CDw137, T-cell antigen 4-1BB homolog, T-cell antigen ILA, CD137

Baculovirus

Human

HIgG-His-Tag

SDS-PAGE, Bioactivity

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Measured by its binding ability in a functional ELISA with Human TNFSF9 (CAT# ATGP0308) . The ED50 range ≤ 50 ng/mL.

45.3 kDa (411aa)

41BB, TNFRSF9, TNFRSF9, Ly63, 4-1BB, ILA, CD137, CDw137, ATGP3303-10 µg, ATGP3303-20 µg, ATGP3303-50 µg, ATGP3303-100 µg, ATGP3303-250 µg, ATGP3303-500 µg, ATGP3303-1 mg, ATGP3303-10, ATGP3303-20, ATGP3303-50, ATGP3303-100, ATGP3303-250, ATGP3303-500, ATGP3303-1

Litjens NH., et al. (2013) Clin. Exp. Immunol. 174:179-191.; ; Lee SJ., et al. (2013) Eur. J. Immunol. 43:1839-1848.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Immunology

NP_001552

Q07011

Human

Ala

FERTRSL QDPCSNCPAG TFCDNNRNQI CSPCPPNSFS SAGGQRTCDI CRQCKGVFRT RKECSSTSNA ECDCTPGFHC LGAGCSMCEQ DCKQGQELTK KGCKDCCFGT FNDQKRGICR PWTNCSLDGK SVLVNGTKER DVVCGPSPAD LSPGASSVTP PAPAREPGHS PQ