Recombinant humaan 4E-BP1/EIF4EBP1-eiwit

EIF4E-BP1, also known as eukaryotic translation initiation factor 4E-binding protein 1, is a member of a family of translation repressor proteins. This protein regulates eukaryotic translation initiation factor 4E (eIF4E) activity by preventing its assembly into the eIF4F complex and mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase and mTORC1 pathways. Recombinant EIF4EBP1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I, MGC4316, Eukaryotic translation initiation factor 4E binding protein 1, EIF4EBP1, EIF4E-binding protein 1, BP-1, 4EBP1

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by BCA assay)

> 95% by SDS-PAGE

14.7 kDa (138aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

Phosphorylated heat- and acid-stable protein regulated by insulin 1, PHAS-I, MGC4316, Eukaryotic translation initiation factor 4E-binding protein 1, Eukaryotic translation initiation factor 4E binding protein 1, EIF4EBP1, EIF4E-binding protein 1, BP-1, 4E-BP1, 4EBP1, ATGP0379-10 µg, ATGP0379-20 µg, ATGP0379-50 µg, ATGP0379-100 µg, ATGP0379-250 µg, ATGP0379-500 µg, ATGP0379-1 mg, ATGP0379-010, ATGP0379-020, ATGP0379-050, ATGP0379-100, ATGP0379-250, ATGP0379-500, ATGP0379-01M

Pause A., et al. (1994) Nature. 371:762-767.; ; Olsen JV., et al. (2006) Cell. 127 (3) :635-48.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Epigenomics (Transcription & Translation)

NP_004086.1

Q13541

Human

MSGGSSCSQT PSRAIPATRR VVLGDGVQLP PGDYSTTPGG TLFSTTPGGT RIIYDRKFLM ECRNSPVTKT PPRDLPTIPG VTSPSSDEPP MEASQSHLRN SPEDKRAGGE ESQFEMDI