Recombinant humaan 4E-BP2/EIF4EBP2-eiwit

EIF4EBP2 is a member of the eukaryotic translation initiation factor 4E binding protein family. This protein binds eIF4E and inhibits translation initiation. However, insulin and other growth factors can release this inhibition via a phosphorylation-dependent disruption of their binding to eIF4E. It mediates the regulation of protein translation by hormones, growth factors and other stimuli that signal through the MAP kinase pathway. Regulation of this protein has been implicated in cell proliferation, cell differentiation and viral infection. Recombinant human EIF4EBP2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Eukaryotic translation initiation factor 4E binding protein 2, 4EBP2, PHASII, eIF4E-binding protein 2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

15.1 kDa (140aa) confirmed by MALDI-TOF

EIF4EBP2, Eukaryotic translation initiation factor 4E binding protein 2, 4EBP2, PHASII, ATGP0964-10 µg, ATGP0964-20 µg, ATGP0964-50 µg, ATGP0964-100 µg, ATGP0964-250 µg, ATGP0964-500 µg, ATGP0964-1 mg, ATGP0964-10, ATGP0964-20, ATGP0964-50, ATGP0964-100, ATGP0964-250, ATGP0964-500, ATGP0964-1

Pause A., et al. (1994) Nature. 371650001) :762-7.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl.

Signal Transduction

NP_004087.1

Q13542

Human

MSSSAGSGHQ PSQSRAIPTR TVAISDAAQL PHDYCTTPGG TLFSTTPGGT RIIYDRKFLL DRRNSPMAQT PPCHLPNIPG VTSPGTLIED SKVEVNNLNN LNNHDRKHAV GDDAQFEMDI