Recombinant humaan 4E-BP3/EIF4EBP3-eiwit

Eukaryotic translation initiation factor 4E-binding protein 3, also known as EIF4EBP 3, is a member of EIF4EBP family which derives its name from proteins that bind to eukaryotic initiation factor 4E and that prevent its assembly into EIF4F. The EIF4F subunit EIF4E interacts directly with the mRNA 5' cap structure. Co-transcription of this gene and the neighboring upstream gene (MASK) generates a transcript (MASK-BP3) which encodes a fusion protein comprised of the MASK protein sequence for the majority of the protein and a different C-terminus due to an alternate reading frame for the EIF4EBP3 segments. Recombinant human EIF4EBP3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Eukaryotic translation initiation factor 4E-binding protein 3, 4E-BP3, 4EBP3, eIF4E-binding protein 3

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

13.3 kDa (123aa) confirmed by MALDI-TOF

EIF4EBP3, Eukaryotic translation initiation factor 4E-binding protein 3, 4E-BP3, 4EBP3, ATGP2772-10 µg, ATGP2772-20 µg, ATGP2772-50 µg, ATGP2772-100 µg, ATGP2772-250 µg, ATGP2772-500 µg, ATGP2772-1 mg, ATGP2772-10, ATGP2772-20, ATGP2772-50, ATGP2772-100, ATGP2772-250, ATGP2772-500, ATGP2772-1

Gingras A C., et al. (1998) Genes Dev. 12: 502-513.; ; Gingras A C., et al. (1999) Genes Dev. 13: 1422-1437

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Epigenomics (Transcription & Translation)

NP_003723

O60516

Human

MGSSHHHHHH SSGLVPRGSH MGSMSTSTSC PIPGGRDQLP DCYSTTPGGT LYATTPGGTR IIYDRKFLLE CKNSPIARTP PCCLPQIPGV TTPPTAPLSK LEELKEQETE EEIPDDAQFE MDI