Recombinant humaan 6-Fosphogluconaatdehydrogenase/PGD-eiwit

PGD (Phosphogluconate dehydrogenas), also known as 6PGD, is a 483 amino acid enzyme that is involved in the pentose phosphate shunt. Pentose is required for nucleic acid biosynthesis and the pentose phosphate cycle is a major source of NADPH. PGD deficiency increases the level of erythrocyte pyruvate kinase (PK) activity and reduces glutathione synthetase (GSH), resulting in hemolysis. Defects in PGD are generally asymptomatic and are inherited in an autosomal dominant fashion. Recombinant human PGD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 10 units/mg, in which one unit oxidize 1.0 umole of 6-phospho-D-gluconate to D-ribulose 5-phosphate per minute at pH 8.0 at 25C, in the presence of beta-NADP.

6-phosphogluconate dehydrogenase, Decarboxylating, PGDH, Phosphogluconate dehydrogenase, 6PGD

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is > 10unit/mg, in which one unit oxidize 1.0 umole of 6-phospho-D-gluconate to D-ribulose 5-phosphate per minute at pH 8.0 at 25C, in the presence of beta-NADP.

55.3 kDa (503aa)

6-Phosphogluconate Dehydrogenase, PGD, Phosphogluconate dehydrogenase, 6PGD, ATGP3238-10 µg, ATGP3238-20 µg, ATGP3238-50 µg, ATGP3238-100 µg, ATGP3238-250 µg, ATGP3238-500 µg, ATGP3238-1 mg, ATGP3238-10, ATGP3238-20, ATGP3238-50, ATGP3238-100, ATGP3238-250, ATGP3238-500, ATGP3238-1

Le TD., et al. (2010) Proc Natl Acad Sci U S A. 107 (7) :3198-203.; ; Tagen M., et al. (2009) J Immunol. 183 (10) :6313-9.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 0.1M Nacl, 10% glycerol

Redox Proteins

NP_002622

P52209

Human

MGSSHHHHHH SSGLVPRGSH MAQADIALIG LAVMGQNLIL NMNDHGFVVC AFNRTVSKVD DFLANEAKGT KVVGAQSLKE MVSKLKKPRR IILLVKAGQA VDDFIEKLVP LLDTGDIIID GGNSEYRDTT RRCRDLKAKG ILFVGSGVSG GEEGARYGPS LMPGGNKEAW PHIKTIFQGI AAKVGTGEPC CDWVGDEGAG HFVKMVHNGI EYGDMQLICE AYHLMKDVLG MAQDEMAQAF EDWNKTELDS FLIEITANIL KFQDTDGKHL LPKIRDSAGQ KGTGKWTAIS ALEYGVPVTL IGEAVFARCL SSLKDERIQA SKKLKGPQKF QFDGDKKSFL EDIRKALYAS KIISYAQGFM LLRQAATEFG WTLNYGGIAL MWRGGCIIRS VFLGKIKDAF DRNPELQNLL LDDFFKSAVE NCQDSWRRAV STGVQAGIPM PCFTTALSFY DGYRHEMLPA SLIQAQRDYF GAHTYELLAK PGQFIHTNWT GHGGTVSSSS YNA