Recombinant humaan a-N-acetylgalactosaminidase-eiwit

NAGA, also known as alpha-N-acetylgalactosaminidase, is a lysosomal exoglycosidase that cleaves terminal alpha-N-acetylgalactosamine residues from glycopeptides and glycolipids. It is hardly expected to cause an allergic reaction in Fabry disease patients. It is highly promising as a new and safe enzyme for ERT for Fabry disease. Recombinant human NAGA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Alpha-N-acetylgalactosaminidase, NAGA, D22S674, GALB, Acetylgalactosaminidase, alpha N (alpha galactosidase B), Alpha galactosidase B, Alpha N acetylgalactosaminidase, N acetylgalactosaminidase, alpha

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

45.5 kDa (400aa)

A-N-acetylgalactosaminidase, Alpha-N-acetylgalactosaminidase, NAGA, D22S674, GALB, Acetylgalactosaminidase, alpha N (alpha galactosidase B), Alpha galactosidase B, Alpha N acetylgalactosaminidase, N acetylgalactosaminidase, alpha, ATGP3377-10 µg, ATGP3377-20 µg, ATGP3377-50 µg, ATGP3377-100 µg, ATGP3377-250 µg, ATGP3377-500 µg, ATGP3377-1 mg, ATGP3377-10, ATGP3377-20, ATGP3377-50, ATGP3377-100, ATGP3377-250, ATGP3377-500, ATGP3377-1

Clark NE., et al. (2009) J Mol Biol. 393:435-447.; ; Tajima Y., et al. (2009) Am J Hum Genet. 85:569-580.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Metabolism

NP_000253

P17050

Human

Leu18