Recombinant humaan ABHD14B eiwit

ABHD14B (abhydrolase domain containing 14B) belongs to the AB hydrolase superfamily. This protein contains an alpha/beta hydrolase fold, which is a catalytic domain found in a very wide range of enzymes. In molecular biology, the alpha/beta hydrolase fold is common to a number of hydrolytic enzymes of widely differing phylogenetic origin and catalytic function. The Ab hydrolase domain containing (ABHD) gene subfamily is comprised of 15 mostly uncharacterized members. ABHD14B has hydrolase activity towards p-nitrophenyl butyrate (in vitro) . It may activate transcription. Recombinant human ABHD14B protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Abhydrolase domain containing 14B, CIB

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

25kDa (234aa) confirmed by MALDI-TOF

ABHD14B, Abhydrolase domain containing 14B, CIB, ATGP1394-10 µg, ATGP1394-20 µg, ATGP1394-50 µg, ATGP1394-100 µg, ATGP1394-250 µg, ATGP1394-500 µg, ATGP1394-1 mg, ATGP1394-10, ATGP1394-20, ATGP1394-50, ATGP1394-100, ATGP1394-250, ATGP1394-500, ATGP1394-1

Padmanabhan B., et al. (2004) J. Biol. Chem. 279:9615-9624; ; Holmquist, M. (2000) Curr. Protein Pept. Sci. 1: 209-235.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 100 mM NaCl

Enzymes & Proteases

NP_116139

Q96IU4

Human

MAASVE QREGTIQVQG QALFFREALP GSGQARFSVL LLHGIRFSSE TWQNLGTLHR LAQAGYRAVA IDLPGLGHSK EAAAPAPIGE LAPGSFLAAV VDALELGPPV VISPSLSGMY SLPFLTAPGS QLPGFVPVAP ICTDKINAAN YASVKTPALI VYGDQDPMGQ TSFEHLKQLP NHRVLIMKGA GHPCYLDKPE EWHTGLLDFL QGLQ