Recombinant humaan ABO-eiwit

ABO, also known as NAGAT, belongs to the glycosyltransferase 6 family. This protein is the basis of the ABO blood group system and related to the first discovered blood group system, ABO. Which allele is present in an individual determines the blood group. The histo-blood group ABO involves three carbohydrate antigens: A, B, and H. A, B, and AB individuals express a glycosyltransferase activity that converts the H antigen to the A antigen (by addition of uDP-GalNAc) or to the B antigen (by addition of uDP-Gal), whereas O individuals lack such activity. Recombinant human ABO protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Histo-blood group ABO system transferase, A3GALNT, A3GALT1, GTB, NAGAT, A transferase, B transferase, Fucosylglycoprotein 3-alpha-galactosyltransferase, alpha 1-3-N-acetylgalactosaminyltransferase, alpha 1-3-galactosyltransferase

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

37.4 kDa (322aa) confirmed by MALDI-TOF

ABO, Histo-blood group ABO system transferase, A3GALNT, A3GALT1, GTB, NAGAT, A transferase, B transferase, Fucosylglycoprotein 3-alpha-galactosyltransferase, alpha 1-3-N-acetylgalactosaminyltransferase, alpha 1-3-galactosyltransferase, ATGP1185-10 µg, ATGP1185-20 µg, ATGP1185-50 µg, ATGP1185-100 µg, ATGP1185-250 µg, ATGP1185-500 µg, ATGP1185-1 mg, ATGP1185-10, ATGP1185-20, ATGP1185-50, ATGP1185-100, ATGP1185-250, ATGP1185-500, ATGP1185-1

Persson M., et al. (2007) J. Biol. Chem. 282:9564-9570; ; Nydegger, u.E., et al. (2005) Ann. N.Y. Acad. Sci. 1050: 40-51.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 2mM DTT, 20% glycerol, 200 mM NaCl

Enzymes & Proteases

NP_065202

P16442

Human

MGSSHHHHHH SSGLVPRGSH MAVREPDHLQ RVSLPRMVYP QPKVLTPCRK DVLVVTPWLA PIVWEGTFNI DILNEQFRLQ NTTIGLTVFA IKKYVAFLKL FLETAEKHFM VGHRVHYYVF TDQPAAVPRV TLGTGRQLSV LEVRAYKRWQ DVSMRRMEMI SDFCERRFLS EVDYLVCVDV DMEFRDHVGV EILTPLFGTL HPGFYGSSRE AFTYERRPQS QAYIPKDEGD FYYLGGFFGG SVQEVQRLTR ACHQAMMVDQ ANGIEAVWHD ESHLNKYLLR HKPTKVLSPE YLWDQQLLGW PAVLRKLRFT AVPKNHQAVR NP