Recombinant humaan ACAA2-eiwit

ACAA2 catalyzes the last step of the mitochondrial fatty acid beta-oxidation spiral. unlike most mitochondrial matrix proteins, it contains a non-cleavable amino-terminal targeting signal. Recombinant human ACAA2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Acetyl-CoA acyltransferase 2, DSAEC

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

42.6 kDa (404aa) confirmed by MALDI-TOF

ACAA, ATGP2020-10 µg, ATGP2020-20 µg, ATGP2020-50 µg, ATGP2020-100 µg, ATGP2020-250 µg, ATGP2020-500 µg, ATGP2020-1 mg, ATGP2020-10, ATGP2020-20, ATGP2020-50, ATGP2020-100, ATGP2020-250, ATGP2020-500, ATGP2020-1

Cao, W , et al. (2008) Biochim. Biophys. Acta 1780 (6), 873-880

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol,1mM DTT

Metabolism

NP_006102

P42765

Human

FGAYGGL LKDFTATDLS EFAAKAALSA GKVSPETVDS VIMGNVLQSS SDAIYLARHV GLRVGIPKET PALTINRLCG SGFQSIVNGC QEICVKEAEV VLCGGTESMS QAPYCVRNVR FGTKLGSDIK LEDSLWVSLT DQHVQLPMAM TAENLAVKHK ISREECDKYA LQSQQRWKAA NDAGYFNDEM APIEVKTKKG KQTMQVDEHA RPQTTLEQLQ KLPPVFKKDG TVTAGNASGV ADGAGAVIIA SEDAVKKHNF TPLARIVGYF VSGCDPSIMG IGPVPAISGA LKKAGLSLKD MDLVEVNEAF APQYLAVERS LDLDISKTNV NGGAIALGHP LGGSGSRITA HLVHELRRRG GKYAVGSACI GGGQGIAVII QSTA