Recombinant humaan ACAD8-eiwit

ACAD8 is a member of the acyl-CoA dehydrogenase family of enzymes that catalyze the dehydrogenation of acyl-CoA derivatives in the metabolism of fatty acids or branch chained amino acids. The protein is a mitochondrial enzyme that functions in catabolism of the branched-chain amino acid valine. Defects in this gene are the cause of isobutyryl-CoA dehydrogenase deficiency. Recombinant human ACAD8 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Isobutyryl-CoA dehydrogenase mitochondrial, isobutyryl-CoA dehydrogenase, mitochondrial, ACAD-8, ARC42, Activator-recruited cofactor 42 kDa component, Acyl-CoA dehydrogenase family member 8, IBD

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

45.1 kDa (416aa) confirmed by MALDI-TOF

ACAD8, isobutyryl-CoA dehydrogenase mitochondrial, isobutyryl-CoA dehydrogenase, mitochondrial, ACAD-8, ARC42, Activator-recruited cofactor 42 kDa component, Acyl-CoA dehydrogenase family member 8, IBD, ATGP2421-10 µg, ATGP2421-20 µg, ATGP2421-50 µg, ATGP2421-100 µg, ATGP2421-250 µg, ATGP2421-500 µg, ATGP2421-1 mg, ATGP2421-10, ATGP2421-20, ATGP2421-50, ATGP2421-100, ATGP2421-250, ATGP2421-500, ATGP2421-1

Hendrickson, S.L., et al. (2010) PLoS ONE 5 (9), E12862; ; Battaile, K.P., et al. (2004) J. Biol. Chem. 279 (16), 16526-16534;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 30% glycerol, 1mM DTT

Metabolism

NP_055199

Q9UKU7

Human

MGSSHHHHHH SSGLVPRGSH MGSLVQTGHR SLTSCIDPSM GLNEEQKEFQ KVAFDFAARE MAPNMAEWDQ KELFPVDVMR KAAQLGFGGV YIQTDVGGSG LSRLDTSVIF EALATGCTST TAYISIHNMC AWMIDSFGNE EQRHKFCPPL CTMEKFASYC LTEPGSGSDA ASLLTSAKKQ GDHYILNGSK AFISGAGESD IYVVMCRTGG PGPKGISCIV VEKGTPGLSF GKKEKKVGWN SQPTRAVIFE DCAVPVANRI GSEGQGFLIA VRGLNGGRIN IASCSLGAAH ASVILTRDHL NVRKQFGEPL ASNQYLQFTL ADMATRLVAA RLMVRNAAVA LQEERKDAVA LCSMAKLFAT DECFAICNQA LQMHGGYGYL KDYAVQQYVR DSRVHQILEG SNEVMRILIS RSLLQE