Recombinant humaan ACADL-eiwit

ACADL belongs to the acyl-CoA dehydrogenase family, existing as a homotetramer. It is involved in the catabolism of fatty acids and amino acids and they provide a major source of energy for the heart and skeletal muscle. Defect in the gene encoding ACADL leads to non-ketotic hypoglycemia and hypotonia, muscle weakness. Recombinant human ACADL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Acyl-CoA dehydrogenase long chain, Acyl-CoA dehydrogenase, long chain, ACAD4, FLJ94052, LCAD

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

46.7 kDa (421aa) confirmed by MALDI-TOF

ACADL, Acyl-CoA dehydrogenase long chain, Acyl-CoA dehydrogenase, long chain, ACAD4, FLJ94052, LCAD, ATGP1278-10 µg, ATGP1278-20 µg, ATGP1278-50 µg, ATGP1278-100 µg, ATGP1278-250 µg, ATGP1278-500 µg, ATGP1278-1 mg, ATGP1278-10, ATGP1278-20, ATGP1278-50, ATGP1278-100, ATGP1278-250, ATGP1278-500, ATGP1278-1

Maher AC., et al. (2010) Mol Genet Metab. 100 (2) :163-7.; ; Wanders RJ., et al. (1998) Biochim Biophys Acta. 1393 (1) :35-40.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl

Cancer

NP_001599

P28330

Human

GGEERLETP SAKKLTDIGI RRIFSPEHDI FRKSVRKFFQ EEVIPHHSEW EKAGEVSREV WEKAGKQGLL GVNIAEHLGG IGGDLYSAAI VWEEQAYSNC SGPGFSIHSG IVMSYITNHG SEEQIKHFIP QMTAGKCIGA IAMTEPGAGS DLQGIKTNAK KDGSDWILNG SKVFISNGSL SDVVIVVAVT NHEAPSPAHG ISLFLVENGM KGFIKGRKLH KMGLKAQDTA ELFFEDIRLP ASALLGEENK GFYYIMKELP QERLLIADVA ISASEFMFEE TRNYVKQRKA FGKTVAHLQT VQHKLAELKT HICVTRAFVD NCLQLHEAKR LDSATACMAK YWASELQNSV AYDCVQLHGG WGYMWEYPIA KAYVDARVQP IYGGTNEIMK ELIAREIVFD K