Recombinant humaan ACADVL-eiwit

ACADVL, also known as VLCAD, LCACD or ACAD6, is an inner mitochondrial membrane protein that belongs to the family of acyl-CoA dehydrogenases. This protein is involved in lipid metabolism and has catalytic activity toward esters of long chain and very long chain fatty acids such as palmitoyl-CoA and stearoyl-CoA, and functions in the first step of the fatty acid beta-oxidation pathway. A deficiency in this gene product reduces myocardial fatty acid beta-oxidation and is associated with cardiomyopathy. Recombinant human ACADVL protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Very long-chain specific acyl-CoA dehydrogenase mitochondrial, ACAD6, LCACD, VLCAD, Very long-chain specific acyl-CoA dehydrogenase, mitochondrial

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

68.5 kDa (636aa)

ACADVL, Very long-chain specific acyl-CoA dehydrogenase mitochondrial, ACAD6, LCACD, VLCAD, Very long-chain specific acyl-CoA dehydrogenase, mitochondrial, ATGP0675-10 µg, ATGP0675-20 µg, ATGP0675-50 µg, ATGP0675-100 µg, ATGP0675-250 µg, ATGP0675-500 µg, ATGP0675-1 mg, ATGP0675-10, ATGP0675-20, ATGP0675-50, ATGP0675-100, ATGP0675-250, ATGP0675-500, ATGP0675-1

Smelt AH., et al. (1998) Ann Neurol. 43 (4) :540-4. ; ; Souri M., et al. (1996) Am J Hum Genet. 58 (1) :97-106. ;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 1mM DTT, 1mM EDTA, 0.1M NaCl.

Enzymes & Proteases

NP_000009

P49748

Human

MAGGAAQLAL DKSDSHPSDA LTRKKPAKAE SKSFAVGMFK GQLTTDQVFP YPSVLNEEQT QFLKELVEPV SRFFEEVNDP AKNDALEMVE ETTWQGLKEL GAFGLQVPSE LGGVGLCNTQ YARLVEIVGM HDLGVGITLG AHQSIGFKGI LLFGTKAQKE KYLPKLASGE TVAAFCLTEP SSGSDAASIR TSAVPSPCGK YYTLNGSKLW ISNGGLADIF TVFAKTPVTD PATGAVKEKI TAFVVERGFG GITHGPPEKK MGIKASNTAE VFFDGVRVPS ENVLGEVGSG FKVAMHILNN GRFGMAAALA GTMRGIIAKA VDHATNRTQF GEKIHNFGLI QEKLARMVML QYVTESMAYM VSANMDQGAT DFQIEAAISK IFGSEAAWKV TDECIQIMGG MGFMKEPGVE RVLRDLRIFR IFEGTNDILR LFVALQGCMD KGKELSGLGS ALKNPFGNAG LLLGEAGKQL RRRAGLGSGL SLSGLVHPEL SRSGELAVRA LEQFATVVEA KLIKHKKGIV NEQFLLQRLA DGAIDLYAMV VVLSRASRSL SEGHPTAQHE KMLCDTWCIE AAARIREGMA ALQSDPWQQE LYRNFKSISK ALVERGGVVT SNPLGF