Recombinant humaan ACAT1-eiwit

ACAT1, also known as acetoacetyl-CoA thiolase, is an enzyme belongs to belongs to the membrane-bound acyltransferase family and Sterol o-acyltransferase subfamily. ACAT1 is an acetyl-CoA C-acetyltransferase enzyme. This enzyme catalyzes the reversible formation of acetoacetyl-CoA from two molecules of acetyl-CoA. It plays a role in lipoprotein assembly and dietary cholesterol absorption. In addition to its acyltransferase activity, it may act as a ligase. Recombinant human ACAT1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Acetyl-CoA acetyltransferase 1, ACAT, MAT, T2, THIL

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

43.8 kDa (417aa) confirmed by MALDI-TOF

ACAT1, acetyl-CoA acetyltransferase 1, ACAT, MAT, T2, THIL, ATGP1800-10 µg, ATGP1800-20 µg, ATGP1800-50 µg, ATGP1800-100 µg, ATGP1800-250 µg, ATGP1800-500 µg, ATGP1800-1 mg, ATGP1800-10, ATGP1800-20, ATGP1800-50, ATGP1800-100, ATGP1800-250, ATGP1800-500, ATGP1800-1

Haapalainen, A.M., et al. (2007) Biochemistry 46: 4305-4321.; ; Thompson, S.L. et al. (1990) J. Biol. Chem. 265:5731-5735.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 0.1M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_000010

P24752

Human

VSKPTLK EVVIVSATRT PIGSFLGSLS LLPATKLGSI AIQGAIEKAG IPKEEVKEAY MGNVLQGGEG QAPTRQAVLG AGLPISTPCT TINKVCASGM KAIMMASQSL MCGHQDVMVA GGMESMSNVP YVMNRGSTPY GGVKLEDLIV KDGLTDVYNK IHMGSCAENT AKKLNIARNE QDAYAINSYT RSKAAWEAGK FGNEVIPVTV TVKGQPDVVV KEDEEYKRVD FSKVPKLKTV FQKENGTVTA ANASTLNDGA AALVLMTADA AKRLNVTPLA RIVAFADAAV EPIDFPIAPV YAASMVLKDV GLKKEDIAMW EVNEAFSLVV LANIKMLEID PQKVNINGGA VSLGHPIGMS GARIVGHLTH ALKQGEYGLA SICNGGGGAS AMLIQKL