Recombinant humaan ACBD6-eiwit

ACBD6 contains 1 ACB (acyl-CoA-binding) domain and 2 ANK repeats. This protein binds long-chain acyl-coenzyme A molecules with a strong preference for unsaturated C18:1 CoA, lower affinity for unsaturated C20:4-CoA, and very weak affinity for saturated C16:0-CoA. It does not bind fatty acids. Recombinant human ASCC1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Acyl-CoA-binding domain-containing protein 6, acyl-CoA binding domain containing 6, MGC2404

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

33.5 kDa (305aa) confirmed by MALDI-TOF

ACBD6, acyl-CoA-binding domain-containing protein 6, acyl-CoA binding domain containing 6, MGC2404, ATGP2385-10 µg, ATGP2385-20 µg, ATGP2385-50 µg, ATGP2385-100 µg, ATGP2385-250 µg, ATGP2385-500 µg, ATGP2385-1 mg, ATGP2385-10, ATGP2385-20, ATGP2385-50, ATGP2385-100, ATGP2385-250, ATGP2385-500, ATGP2385-1

Soupene E., et al (2008) . J. Lipid Res. 49:1103-1112; ; Burkard T.R., et al (2011) . BMC Syst. Biol. 5:17-17;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 20% glycerol, 1mM DTT

Metabolism

NP_115736

Q9BR61

Human

MGSSHHHHHH SSGLVPRGSH MGSMASSFLP AGAITGDSGG ELSSGDDSGE VEFPHSPEIE ETSCLAELFE KAAAHLQGLI QVASREQLLY LYARYKQVKV GNCNTPKPSF FDFEGKQKWE AWKALGDSSP SQAMQEYIAV VKKLDPGWNP QIPEKKGKEA NTGFGGPVIS SLYHEETIRE EDKNIFDYCR ENNIDHITKA IKSKNVDVNV KDEEGRALLH WACDRGHKEL VTVLLQHRAD INCQDNEGQT ALHYASACEF LDIVELLLQS GADPTLRDQD GCLPEEVTGC KTVSLVLQRH TTGKA