Recombinant humaan ACE-2-eiwit

ACE-2, also known as Angiotensin-converting enzyme 2, is an enzyme attached to the cell membranes of cells in the lungs, arteries, heart, kidney, and intestines. This enzyme lowers blood pressure by catalysing the hydrolysis of angiotensin II into angiotensin. It counters the activity of the related angiotensin-converting enzyme (ACE) by reducing the amount of angiotensin-II and increasing Ang (1-7) making it a promising drug target for treating cardiovascular diseases. ACE-2 also serves as the entry point into cells for some coronaviruses, including HCoV-NL63, SARS-CoV, and SARS-CoV-2. Recombinant human ACE-2, fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques

SARS-CoV-2 Spike RBD is coated at 5 µg/mL (100 ul/well) can bind Human ACE-2. The ED50 range ≤ 100 ng/mL.

Angiotensin-converting enzyme 2, ACEH, Angiotensin-converting enzyme-related carboxypeptidase, ACE-related carboxypeptidase, Metalloprotease MPROT15, ACE2

HEK293

Human

HIgG-His-Tag

SDS-PAGE, Enzyme Activity, Bioactivity

0.5 mg/mL (determined by Absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS - PAGE

Measured by its binding ability in a functional ELISA with SARS-CoV-2 Spike RBD. The ED50 range ≤ 100 ng/mL. Specific activity is > 250 pmol/min/ug, and is defined as the amount of enzyme that hydrolysis 1.0 pmole of Mca-YVADAPK (Dnp) -OH per minute at pH 7.5, at 25C.

110.8kDa (965aa)

, ATGP3963-10 µg, ATGP3963-20 µg, ATGP3963-50 µg, ATGP3963-100 µg, ATGP3963-250 µg, ATGP3963-500 µg, ATGP3963-1 mg, ATGP3963-10, ATGP3963-20, ATGP3963-50, ATGP3963-100, ATGP3963-250, ATGP3963-500, ATGP3963-1

Hamming I., et al, (2004) J Pathol. 203:631-637.; ; Mohammed A.r. Chamsi-Pasha., et al, (2014) Curr Heart Fail Rep. 11:58-63.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid. In Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Virus

NP_068576.1

Q9BYF1

Human

Asp

QSTIEEQ AKTFLDKFNH EAEDLFYQSS LASWNYNTNI TEENVQNMNN AGDKWSAFLK EQSTLAQMYP LQEIQNLTVK LQLQALQQNG SSVLSEDKSK RLNTILNTMS TIYSTGKVCN PDNPQECLLL EPGLNEIMAN SLDYNERLWA WESWRSEVGK QLRPLYEEYV VLKNEMARAN HYEDYGDYWR GDYEVNGVDG YDYSRGQLIE DVEHTFEEIK PLYEHLHAYV RAKLMNAYPS YISPIGCLPA HLLGDMWGRF WTNLYSLTVP FGQKPNIDVT DAMVDQAWDA QRIFKEAEKF FVSVGLPNMT QGFWENSMLT DPGNVQKAVC HPTAWDLGKG DFRILMCTKV TMDDFLTAHH EMGHIQYDMA YAAQPFLLRN GANEGFHEAV GEIMSLSAAT PKHLKSIGLL SPDFQEDNET EINFLLKQAL TIVGTLPFTY MLEKWRWMVF KGEIPKDQWM KKWWEMKREI VGVVEPVPHD ETYCDPASLF HVSNDYSFIR YYTRTLYQFQ FQEALCQAAK HEGPLHKCDI SNSTEAGQKL FNMLRLGKSE PWTLALENVV GAKNMNVRPL LNYFEPLFTW LKDQNKNSFV GWSTDWSPYA DQSIKVRISL KSALGDKAYE WNDNEMYLFR SSVAYAMRQY FLKVKNQMIL FGEEDVRVAN LKPRISFNFF VTAPKNVSDI IPRTEVEKAI RMSRSRINDA FRLNDNSLEF LGIQPTLGPP NQPPVS