Recombinant humaan ACE-eiwit

ACE, also known as peptidyl-dipetidase A, is a zinc metallopeptidase important for blood pressure control and water and salt metabolism. it converts angiotensin I to angiotensin II by release of the terminal His-Leu, this results in an increase of the vasoconstrictor activity of angiotensin. It's able to inactivate bradykinin, a potent vasodilator. It has also a glycosidase activity which releases GPI-anchored proteins from the membrane by cleaving the mannose linkage in the GPI moiety. Recombinant human ACE/CD143, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Specific activity is > 1,000 pmol/min/mg, and is defined as the amount of enzyme that cleaves 1pmol of Mca-RPPGFSAFK (Dnp) -OH per minute at 25C.

Angiotensin-coverting enzyme, Dipeptidyl carboxypeptidase I, Kininase II, ACE1, CD143, DCP, DCP1

Baculovirus

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.25 mg/mL (determined by Absorbance at 280 nm)

<1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is > 1,000 pmol/min/mg, and is defined as the amount of enzyme that cleaves 1pmol of Mca-RPPGFSAFK (Dnp) -OH per minute at 25C.

142kDa (1235aa)

, ATGP3944-10 µg, ATGP3944-20 µg, ATGP3944-50 µg, ATGP3944-100 µg, ATGP3944-250 µg, ATGP3944-500 µg, ATGP3944-1 mg, ATGP3944-10, ATGP3944-20, ATGP3944-50, ATGP3944-100, ATGP3944-250, ATGP3944-500, ATGP3944-1

Amati E., et al, (2018) Stem Cell Res Ther. 9:10.; ; Metzger R., et al, (2000) Atherosclerosis. 150:21-31.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cardiovascular

NP_000780

P12821

Human

Leu 30