Recombinant humaan ACOT11-eiwit

BFIT is a member of the acyl-CoA thioesterase family which catalyse the conversion of activated fatty acids to the corresponding non-esterified fatty acid and coenzyme A. Expression of a mouse homolog in brown adipose tissue is induced by low temperatures and repressed by warm temperatures. Higher levels of expression of the mouse homolog has been found in obesity-resistant mice compared with obesity-prone mice, suggesting a role of acyl-CoA thioesterase 11 in obesity. The protein has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Recombinant human BFIT protein, fused to His-tag at N-terminus, was expressed in E. coli

Thioesterase adipose associated isoform BFIT2, Acyl-CoA thioesterase 11, THEA, STASTARD14, BFIT, KIAA0707, BFIT1, THEM1

E.coli

Human

His-Tag

SDS-PAGE, Denatured

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

29.9 kDa (268aa)

BFIT, Thioesterase adipose associated isoform BFIT2, Thioesterase, adipose associated isoform BFIT2, ATGP2436-10 µg, ATGP2436-20 µg, ATGP2436-50 µg, ATGP2436-100 µg, ATGP2436-250 µg, ATGP2436-500 µg, ATGP2436-1 mg, ATGP2436-10, ATGP2436-20, ATGP2436-50, ATGP2436-100, ATGP2436-250, ATGP2436-500, ATGP2436-1

Thorsell AG, Lee WH., et al. (2011) Plos One 6 p.19521

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.4M urea, 10% glycerol

Metabolism

NP_671517

Q8WXI4

Human

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSNRTS RKSALRAGND SAMADGEGYR NPTEVQMSQL VLPCHTNQRG ELSVGQLLKW IDTTACLSAE RHAGCPCVTA SMDDIYFEHT ISVGQVVNIK AKVNRAFNSS MEVGIQVASE DLCSEKQWNV CKALATFVAR REITKVKLKQ ITPRTEEEKM EHSVAAERRR MRLVYADTIK DLLANCAIQG DLESRDCSRM VPAEKTRVES VELVLPPHAN HQGNTFGGQI MAWMENVA