Recombinant humaan ACOT13-eiwit

Acyl-coenzyme A thioesterase 13, also known ACOT13, belongs to the thioesterase subfamily of esterase family. Highly expressed in kidney with moderate expression in brain, liver and intestines, ACOT13 contains a hotdog-fold and is thought to co-localize with microtubules, possibly playing a role in cellular proliferation events. Deletion of a portion of the q arm of chromosome 6 is associated with early onset intestinal cancer, suggesting the presence of a cancer susceptibility locus. Recombinant human ACOT13, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Acyl-coenzyme A thioesterase 13, THEM2, HT012, PNAS-27, Acyl-coenzyme A thioesterase 13

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

17.1 kDa (160aa) confirmed by MALDI-TOF

ACOT13, Acyl-coenzyme A thioesterase 13, THEM2, HT012, PNAS-27, Acyl-coenzyme A thioesterase 13, ATGP0731-10 µg, ATGP0731-20 µg, ATGP0731-50 µg, ATGP0731-100 µg, ATGP0731-250 µg, ATGP0731-500 µg, ATGP0731-1 mg, ATGP0731-10, ATGP0731-20, ATGP0731-50, ATGP0731-100, ATGP0731-250, ATGP0731-500, ATGP0731-1

Kanno K., et al. (2007) J Biol Chem. 282:30728-30736.; ; Cheng Z., et al. (2006) Biochem Biophys Res Commun. 350:850-853.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol

Cytoskeleton

NP_060943

Q9NPJ3

Human

MTSMTQSLRE VIKAMTKARN FERVLGKITL VSAAPGKVIC EMKVEEEHTN AIGTLHGGLT ATLVDNISTM ALLCTERGAP GVSVDMNITY MSPAKLGEDI VITAHVLKQG KTLAFTSVDL TNKATGKLIA QGRHTKHLGN