Recombinant humaan ACOT7-eiwit

ACOT7 is a member of the acyl coenzyme family. This protein hydrolyzes the CoA thioester of palmitoyl-CoA and other long-chain fatty acids. Decreased expression of this protein may be associated with mesial temporal lobe epilepsy. Alternatively spliced transcript variants encoding distinct isoforms with different subcellular locations have been characterized. Recombinant human ACOT7 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Acyl-CoA thioesterase 7, ACH1, ACT, BACH, CTE-II, hBACH, LACH, LACH1, MGC1126, RP1-120G22

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

42.6 kDa (390aa) confirmed by MALDI-TOF

ACOT7, Acyl-CoA thioesterase 7, ACH1, ACT, BACH, CTE-II, hBACH, LACH, LACH1, MGC1126, RP1-120G22, ATGP1340-10 µg, ATGP1340-20 µg, ATGP1340-50 µg, ATGP1340-100 µg, ATGP1340-250 µg, ATGP1340-500 µg, ATGP1340-1 mg, ATGP1340-10, ATGP1340-20, ATGP1340-50, ATGP1340-100, ATGP1340-250, ATGP1340-500, ATGP1340-1

Hunt MC., et al. (2005) J Lipid Res. 46, 2029-32.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.15M NaCl.

Neuroscience

NP_009205

O00154

Human

MARPGLIHSA PGLPDTCALL QPPAASAAAA PSMSGPDVET PSAIQICRIM RPDDANVAGN VHGGTILKMI EEAGAIISTR HCNSQNGERC VAALARVERT DFLSPMCIGE VAHVSAEITY TSKHSVEVQV NVMSENILTG AKKLTNKATL WYVPLSLKNV DKVLEVPPVV YSRQEQEEEG RKRYEAQKLE RMETKWRNGD IVQPVLNPEP NTVSYSQSSL IHLVGPSDCT LHGFVHGGVT MKLMDEVAGI VAARHCKTNI VTASVDAINF HDKIRKGCVI TISGRMTFTS NKSMEIEVLV DADPVVDSSQ KRYRAASAFF TYVSLSQEGR SLPVPQLVPE TEDEKKRFEE GKGRYLQMKA KRQGHAEPQP