Recombinant humaan ACP6-eiwit

ACP6 also known as Iysophosphatidic acid phosphatase type 6, is Hydrolyzes lysophosphatidic acid (LPA) containing a medium length fatty acid chain to the corresponding monoacylglycerol. Has highest activity with lysophosphatidic acid containing myristate (C14:0), monounsaturated oleate (C18:1) or palmitate (C16:0), and lower activity with C18:0 and C6:0 lysophosphatidic acid. Recombinant human ACP6 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 70 units/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C.

Lysophosphatidic acid phosphatase type 6, lysophosphatidic acid phosphatase type 6, ACPL1, LPAP, PACPL1

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 1,000 unit/mg, and is defined as the amount of enzyme that hydrolyze 1.0 nmole of p-nitrophenyl phosphate (pNPP) per minute at pH 5.0 at 37C.

47.7 kDa (419aa)

ACP6, lysophosphatidic acid phosphatase type 6, lysophosphatidic acid phosphatase type 6, ACPL1, LPAP, PACPL1, ATGP3015-10 µg, ATGP3015-20 µg, ATGP3015-50 µg, ATGP3015-100 µg, ATGP3015-250 µg, ATGP3015-500 µg, ATGP3015-1 mg, ATGP3015-10, ATGP3015-20, ATGP3015-50, ATGP3015-100, ATGP3015-250, ATGP3015-500, ATGP3015-1

Hiroyama M., et al. (1999) J. Biol. Chem. 274:29172-29180.; ; Li J., et al. (2013) Protein Cell 4:548-561.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 30% glycerol, 1mM DTT

Signal Transduction

NP_057445.4

Q9NPH0

Human

ELQEADG QCPVDRSLLK LKMVQVVFRH GARSPLKPLP LEEQVEWNPQ LLEVPPQTQF DYTVTNLAGG PKPYSPYDSQ YHETTLKGGM FAGQLTKVGM QQMFALGERL RKNYVEDIPF LSPTFNPQEV FIRSTNIFRN LESTRCLLAG LFQCQKEGPI IIHTDEADSE VLYPNYQSCW SLRQRTRGRR QTASLQPGIS EDLKKVKDRM GIDSSDKVDF FILLDNVAAE QAHNLPSCPM LKRFARMIEQ RAVDTSLYIL PKEDRESLQM AVGPFLHILE SNLLKAMDSA TAPDKIRKLY LYAAHDVTFI PLLMTLGIFD HKWPPFAVDL TMELYQHLES KEWFVQLYYH GKEQVPRGCP DGLCPLDMFL NAMSVYTLSP EKYHALCSQT QVMEVGNEE