Recombinant humaan ACY3-eiwit

Aspartoacylase 3, also known as ACY3, is member of the Aspartoacylase subfamily. It plays an important role in deacetylating mercapturic acids in kidney proximal tubules. ACY3 localizes to the cytoplasm of S2 and S3 proximal tubules and to the apical domain of S1 proximal tubules. ACY3 protein is also expressed at low levels in stomach, testis, heart, brain, lung and liver, and may function as an HCV (Hepatitis C virus) core binding protein. Recombinant human ACY3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Aspartoacylase 3, ACY-3, HCBP1, Aminoacylase-3

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

37.6 kDa (342aa) confirmed by MALDI-TOF

ACY3, Aspartoacylase 3, ACY-3, HCBP1, Aminoacylase-3, ATGP1136-10 µg, ATGP1136-20 µg, ATGP1136-50 µg, ATGP1136-100 µg, ATGP1136-250 µg, ATGP1136-500 µg, ATGP1136-1 mg, ATGP1136-10, ATGP1136-20, ATGP1136-50, ATGP1136-100, ATGP1136-250, ATGP1136-500, ATGP1136-1

Chen Y R., et al. (2009) J Gastroenterol Hepatol. 24 (7) :1300-4.; ; Pushkin A., et al. (2004) . Am J Physiol Cell Physiol. 286 (4) :C848-56.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 0.1M NaCl

Metabolism

NP_542389

Q96HD9

Human

MCSLPVP REPLRRVAVT GGTHGNEMSG VYLARHWLHA PAELQRASFS AVPVLANPAA TSGCRRYVDH DLNRTFTSSF LNSRPTPDDP YEVTRARELN QLLGPKASGQ AFDFVLDLHN TTANMGTCLI AKSSHEVFAM HLCRHLQLQY PELSCQVFLY QRSGEESYNL DSVAKNGLGL ELGPQPQGVL RADIFSRMRT LVATVLDFIE LFNQGTAFPA FEMEAYRPVG VVDFPRTEAG HLAGTVHPQL QDRDFQPLQP GAPIFQMFSG EDLLYEGEST VYPVFINEAA YYEKGVAFVQ TEKFTFTVPA MPALTPAPSP AS