Recombinant humaan ACYP1-eiwit

ACYP1, also known as erythrocyte acylphosphatase, is a cytosolic enzyme that catalyzes the hydrolysis of the carboxyl-phosphate bond of acylphosphates. Two acylphophatase isoenzymes exist: ACYP1 and ACYP2. The two isoenzymes share 60% homology and have the same substrate specificity, although ACYP1 has higher catalytic activity than ACYP2. Recombinant human ACYP1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Acylphosphatase-1, ACYPE, erythrocyte acylphosphatase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by BCA assay)

> 90% by SDS-PAGE

13.6 kDa (122aa) confirmed by MALDI-TOF

ACYP1, Acylphosphatase-1, ACYPE, erythrocyte acylphosphatase, ATGP0928-10 µg, ATGP0928-20 µg, ATGP0928-50 µg, ATGP0928-100 µg, ATGP0928-250 µg, ATGP0928-500 µg, ATGP0928-1 mg, ATGP0928-10, ATGP0928-20, ATGP0928-50, ATGP0928-100, ATGP0928-250, ATGP0928-500, ATGP0928-1

Fiaschi T, et al. (1995) . FEBS Lett. 367:145-8.; ; Paoli P., et al. (2003) . J. Biol. Chem. 278: 194-199.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl, 1mM DTT

Metabolism

NP_001098

P07311

Human

MGSSHHHHHH SSGLVPRGSH MGSMAEGNTL ISVDYEIFGK VQGVFFRKHT QAEGKKLGLV GWVQNTDRGT VQGQLQGPIS KVRHMQEWLE TRGSPKSHID KANFNNEKVI LKLDYSDFQI VK