Recombinant humaan ADAT2-eiwit

Adenosine deaminase (ADA), also known as ADAT2 is an enzyme involved in purine metabolism. It is needed for the breakdown of adenosine from food and for the turnover of nucleic acids in tissues. Also, ADAT2 is thought to participate in the deamination of adenosine-34 to inosine in many tRNAs. Belonging to the cytidine and deoxycytidylate deaminase protein family, ADAT2 employs zinc as a cofactor. ADAT2 is a 191 amino acid protein that exists as two isoforms produced by alternative splicing events. Recombinant human ADAT2 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

TRNA-specific adenosine deaminase 2, DEADC1, TAD2

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

23.2 kDa (211aa) confirmed by MALDI-TOF

ADAT2, tRNA-specific adenosine deaminase 2, DEADC1, TAD2, ATGP0709-10 µg, ATGP0709-20 µg, ATGP0709-50 µg, ATGP0709-100 µg, ATGP0709-250 µg, ATGP0709-500 µg, ATGP0709-1 mg, ATGP0709-10, ATGP0709-20, ATGP0709-50, ATGP0709-100, ATGP0709-250, ATGP0709-500, ATGP0709-1

Persico AM, et al. (2000) Am. J. Med. Genet. 96 (6) : 784-90.; ; Kimura, K., et al. (2006) Genome Res. 16: 55-65.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 50 mM NaCl

Enzymes & Proteases

NP_872309

Q7Z6V5

Human

MGSSHHHHHH SSGLVPRGSH MEAKAAPKPA ASGACSVSAE ETEKWMEEAM HMAKEALENT EVPVGCLMVY NNEVVGKGRN EVNQTKNATR HAEMVAIDQV LDWCRQSGKS PSEVFEHTVL YVTVEPCIMC AAALRLMKIP LVVYGCQNER FGGCGSVLNI ASADLPNTGR PFQCIPGYRA EEAVEMLKTF YKQENPNAPK SKVRKKECQK S