Recombinant humaan Adenosine Deaminase/ADA-eiwit

ADA, also known as adenosine deaminase, catalyzes the hydrolytic deamination of adenosine and 2-deoxyadenosine. This protein plays an important role in purine metabolism and in adenosine homeostasis. ADA acts as a positive regulator of T-cell coactivation, by binding DPP4. Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion. Recombinant human ADA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity is >40 units/mg, and is defined as the amount of enzyme that convert 1.0 umol of adenosine to inosine per minute at pH 7.5 at 25C.

Adenosine deaminase, Adenosine aminohydrolase, ADA1

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

Specific activity is >40unit/mg, and is defined as the amount of enzyme that convert 1.0 umol of adenosine to inosine per minute at pH 7.5 at 25C.

42.9 kDa (383aa) confirmed by MALDI-TOF

Adenosine Deaminase, ADA, Adenosine deaminase, Adenosine aminohydrolase, ADA1, ATGP1116-10 µg, ATGP1116-20 µg, ATGP1116-50 µg, ATGP1116-100 µg, ATGP1116-250 µg, ATGP1116-500 µg, ATGP1116-1 mg, ATGP1116-10, ATGP1116-20, ATGP1116-50, ATGP1116-100, ATGP1116-250, ATGP1116-500, ATGP1116-1

Gines S., et al. (2002) Biochem. 361:203-209; ; Daddona P.E., et al. (1984) J. Biol. Chem. 259:12101-12106

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 1mM DTT

Enzymes & Proteases

NP_000013.2

P00813

Human

MAQTPAFDKP KVELHVHLDG SIKPETILYY GRRRGIALPA NTAEGLLNVI GMDKPLTLPD FLAKFDYYMP AIAGCREAIK RIAYEFVEMK AKEGVVYVEV RYSPHLLANS KVEPIPWNQA EGDLTPDEVV ALVGQGLQEG ERDFGVKARS ILCCMRHQPN WSPKVVELCK KYQQQTVVAI DLAGDETIPG SSLLPGHVQA YQEAVKSGIH RTVHAGEVGS AEVVKEAVDI LKTERLGHGY HTLEDQALYN RLRQENMHFE ICPWSSYLTG AWKPDTEHAV IRLKNDQANY SLNTDDPLIF KSTLDTDYQM TKRDMGFTEE EFKRLNINAA KSSFLPEDEK RELLDLLYKA YGMPPSASAG QNL