Recombinant humaan Adenosine Kinase/ADK-eiwit

Adenosine kinase, also known as ADK is an abundant enzyme in mammalian tissues that catalyzes the transfer of the gamma-phosphate from ATP to adenosine, thereby serving as a regulator of concentrations of both extracellular adenosine and intracellular adenine nucleotides. Adenosine kinase has widespread effects on the cardiovascular, nervous, respiratory, and immune systems and inhibitors of the enzyme could play an important pharmacological role in increasing intravascular adenosine concentrations and acting as anti-inflammatory agents. Recombinant human Adenosine kinase, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 30 pmol/min/ug and is defined as the amount of enzyme that convert 1.0 pmole of adenosine to AMP per minute at pH 7.5 at 37C in a couple system with PK and LDH.

ADK, AK, Adenosine kinase, Adenosine 5'-phosphotransferase

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 30pmol/min/ug and is defined as the amount of enzyme that convert 1.0pmole of adenosine to AMP per minute at pH 7.5 at 37C in a couple system with PK and LDH.

40.5 kDa (362aa) confirmed by MALDI-TOF

Adenosine Kinase, ADK, ADK, AK, ATGP3713-10 µg, ATGP3713-20 µg, ATGP3713-50 µg, ATGP3713-100 µg, ATGP3713-250 µg, ATGP3713-500 µg, ATGP3713-1 mg, ATGP3713-10, ATGP3713-20, ATGP3713-50, ATGP3713-100, ATGP3713-250, ATGP3713-500, ATGP3713-1

Chakraborty, A., et al. (2002) J. Biol. Chem. 277:47451-47451.; ; Sakowicz M., et al. (2001) Acta Biochim Pol. 48 (3) :745-54.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol, 1mM EDTA, 50 mM NaCl

Signal Transduction

NP_006712

P55263

Human

MGSSHHHHHH SSGLVPRGSH MRENILFGMG NPLLDISAVV DKDFLDKYSL KPNDQILAED KHKELFDELV KKFKVEYHAG GSTQNSIKVA QWMIQQPHKA ATFFGCIGID KFGEILKRKA AEAHVDAHYY EQNEQPTGTC AACITGDNRS LIANLAAANC YKKEKHLDLE KNWMLVEKAR VCYIAGFFLT VSPESVLKVA HHASENNRIF TLNLSAPFIS QFYKESLMKV MPYVDILFGN ETEAATFARE QGFETKDIKE IAKKTQALPK MNSKRQRIVI FTQGRDDTIM ATESEVTAFA VLDQDQKEII DTNGAGDAFV GGFLSQLVSD KPLTECIRAG HYAASIIIRR TGCTFPEKPD FH