Recombinant humaan Adenosylhomocysteïnease/AHCY-eiwit

AHCY, also known as adenosylhomocysteinase, is an enzyme that catalyzes the reversible hydrolysis of S-adenosylhomocysteine (AdoHcy) to adenosine (Ado) and L-homocysteine (Hcy) . It regulates the intracellular S-adenosylhomocysteine (SAH) concentration thought to be important for transmethylation reactions. Deficiency in this protein is one of the different causes of hypermethioninemia. Recombinant human AHCY protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques

Adenosylhomocysteinase isoform 1, AHCY, AdoHcyase, SAHH, S-adenosylhomocysteine hydrolase

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

49.8 kDa (452aa) confirmed by MALDI-TOF

Adenosylhomocysteinease, AHCY, ATGP0606-10 µg, ATGP0606-20 µg, ATGP0606-50 µg, ATGP0606-100 µg, ATGP0606-250 µg, ATGP0606-500 µg, ATGP0606-1 mg, ATGP0606-10, ATGP0606-20, ATGP0606-50, ATGP0606-100, ATGP0606-250, ATGP0606-500, ATGP0606-1

Hershfield MS, et al. (1978) Science 202 (4369) :757-60; ; Gellekink H, et al. (2004) Eur J Hum Genet.. 12 (11) :942-8.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 20% glycerol

Enzymes & Proteases

NP_000678.1

P23526

Human

MSDKLPYKVA DIGLAAWGRK ALDIAENEMP GLMRMRERYS ASKPLKGARI AGCLHMTVET AVLIETLVTL GAEVQWSSCN IFSTQDHAAA AIAKAGIPVY AWKGETDEEY LWCIEQTLYF KDGPLNMILD DGGDLTNLIH TKYPQLLPGI RGISEETTTG VHNLYKMMAN GILKVPAINV NDSVTKSKFD NLYGCRESLI DGIKRATDVM IAGKVAVVAG YGDVGKGCAQ ALRGFGARVI ITEIDPINAL QAAMEGYEVT TMDEACQEGN IFVTTTGCID IILGRHFEQM KDDAIVCNIG HFDVEIDVKW LNENAVEKVN IKPQVDRYRL KNGRRIILLA EGRLVNLGCA MGHPSFVMSN SFTNQVMAQI ELWTHPDKYP VGVHFLPKKL DEAVAEAHLG KLNVKLTKLT EKQAQYLGMS CDGPFKPDHY RY