Recombinant humaan ADH5-eiwit

ADH5 is a member of the alcohol dehydrogenase family. Members of this family metabolize a wide variety of substrates, including ethanol, retinol, other aliphatic alcohols, hydroxysteroids, and lipid peroxidation products. It has virtually no activity for ethanol oxidation, but exhibits high activity for oxidation of long-chain primary alcohols and for oxidation of S-hydroxymethyl-glutathione, a spontaneous adduct between formaldehyde and glutathione. This enzyme is an important component of cellular metabolism for the elimination of formaldehyde, a potent irritant and sensitizing agent that causes lacrymation, rhinitis, pharyngitis, and contact dermatitis. Recombinant human ADH5 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Alcohol dehydrogenase class-3, ADHX, FDH

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

42.3 kDa (398aa) confirmed by MALDI-TOF

ADH5, Alcohol dehydrogenase class-3, ADHX, FDH, ATGP1471-10 µg, ATGP1471-20 µg, ATGP1471-50 µg, ATGP1471-100 µg, ATGP1471-250 µg, ATGP1471-500 µg, ATGP1471-1 mg, ATGP1471-10, ATGP1471-20, ATGP1471-50, ATGP1471-100, ATGP1471-250, ATGP1471-500, ATGP1471-1

Zhang, X. et al. (2010) Carcinogenesis 31 (12), 2118-2123; ; Shimada, M. et al. (2010) Hum. Genet. 128 (4), 433-441

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 7.5) containing 20% glycerol 0.1M Nacl, 1mM DTT

Signal Transduction

NP_000662

P11766

Human

MANEVI KCKAAVAWEA GKPLSIEEIE VAPPKAHEVR IKIIATAVCH TDAYTLSGAD PEGCFPVILG HEGAGIVESV GEGVTKLKAG DTVIPLYIPQ CGECKFCLNP KTNLCQKIRV TQGKGLMPDG TSRFTCKGKT ILHYMGTSTF SEYTVVADIS VAKIDPLAPL DKVCLLGCGI STGYGAAVNT AKLEPGSVCA VFGLGGVGLA VIMGCKVAGA SRIIGVDINK DKFARAKEFG ATECINPQDF SKPIQEVLIE MTDGGVDYSF ECIGNVKVMR AALEACHKGW GVSVVVGVAA SGEEIATRPF QLVTGRTWKG TAFGGWKSVE SVPKLVSEYM SKKIKVDEFV THNLSFDEIN KAFELMHSGK SIRTVVKI