Recombinant humaan ADI1-eiwit

ADI1 is an enzyme that belongs to the acireductone dioxygenase family of metal-binding enzymes, which are involved in methionine salvage. This enzyme may regulate mRNA processing in the nucleus, and may carry out different functions depending on its localization. Also, it down-regulates cell migration mediated by MMP14. Recombinant human ADI1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Acireductone dioxygenase 1, APL1, ARD, FLJ10913, HMFT1638, MTCBP-1, SIPL

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

25.6 kDa (216aa) confirmed by MALDI-TOF

ADI1, Acireductone dioxygenase 1, APL1, ARD, FLJ10913, HMFT1638, MTCBP-1, SIPL, ATGP1975-10 µg, ATGP1975-20 µg, ATGP1975-50 µg, ATGP1975-100 µg, ATGP1975-250 µg, ATGP1975-500 µg, ATGP1975-1 mg, ATGP1975-10, ATGP1975-20, ATGP1975-50, ATGP1975-100, ATGP1975-250, ATGP1975-500, ATGP1975-1

Gotoh, I., et al. (2007) Genes Cells 12 (1), 105-117

Bae DH, et al. Acireductone dioxygenase 1 (ADI1) is regulated by cellular iron by a mechanism involving the iron chaperone, PCBP1, with PCBP2 acting as a potential co-chaperone. (Biochim Biophys Acta Mol Basis Dis. 2020) {https://pubmed.ncbi.nlm.nih.gov/32480040/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 0.15M NaCl, 10% glycerol, 1mM DTT

Metabolism

NP_060739

Q9BV57

Human

MRGSHHHHHH GMASMTGGQQ MGRDLYDDDD KDRWGSSMVL AWYMDDAPGD PRQPHRPDPG RPVGLEQLRR LGVLYWKLDA DKYENDPELE KIRRERNYSW MDIITICKDK LPNYEEKIKM FYEEHLHLDD EIRYILDGSG YFDVRDKEDQ WIRIFMEKGD MVTLPAGIYH RFTVDEKNYT KAMRLFVGEP VWTAYNRPAD HFEARGQYVK FLAQTA