Recombinant humaan ADPRH-eiwit

ADPRH (ADP-ribosylarginine hydrolase), also known as ADP-ribose-L-arginine cleaving enzyme, belongs to the ADP-ribosylglycohydrolase family. This protein catalyzes removal of mono-ADP-ribose from arginine residues of proteins in the ADP-ribosylation cycle. unlike the rat and mouse enzymes, which require DTT for maximal activity, the human enzyme is DTT-independent. Recombinant human ADPRH protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Protein ADP-ribosylarginine hydrolase, ARH1

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

42.1 kDa (381aa) confirmed by MALDI-TOF

ADPRH, Protein ADP-ribosylarginine hydrolase, ARH1, ATGP1633-10 µg, ATGP1633-20 µg, ATGP1633-50 µg, ATGP1633-100 µg, ATGP1633-250 µg, ATGP1633-500 µg, ATGP1633-1 mg, ATGP1633-10, ATGP1633-20, ATGP1633-50, ATGP1633-100, ATGP1633-250, ATGP1633-500, ATGP1633-1

Weber F, et al. (2005) J Clin Endocrinol Metab. 90 (2) :1149-55.; ; Kernstock S, et al. (2009) Acta Crystallogr Sect F Struct Biol Cryst Commun. 65 (Pt 5) :529-32.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 100 mM NaCl

Epigenomics (Transcription & Translation)

NP_001116

P54922

Human

MGSSHHHHHH SSGLVPRGSH MGSHMEKYVA AMVLSAAGDA LGYYNGKWEF LQDGEKIHRQ LAQLGGLDAL DVGRWRVSDD TVMHLATAEA LVEAGKAPKL TQLYYLLAKH YQDCMEDMDG RAPGGASVHN AMQLKPGKPN GWRIPFNSHE GGCGAAMRAM CIGLRFPHHS QLDTLIQVSI ESGRMTHHHP TGYLGALASA LFTAYAVNSR PPLQWGKGLM ELLPEAKKYI VQSGYFVEEN LQHWSYFQTK WENYLKLRGI LDGESAPTFP ESFGVKERDQ FYTSLSYSGW GGSSGHDAPM IAYDAVLAAG DSWKELAHRA FFHGGDSDST AAIAGCWWGV MYGFKGVSPS NYEKLEYRNR LEETARALYS LGSKEDTVIS L