Recombinant humaan AES-eiwit

Amino-terminal enhancer of split, also known AES, belongs to the groucho/TLE family of proteins, can function as a homooligomer or as a heteroologimer with other family members to dominantly repress the expression of other family member genes. This protein is expressed predominately in fetal brain, liver, lung, heart and kidney and in adult muscle. In addition, AES can repress NFkB-regulated gene expression and is thought to play an important role in initiating and maintaining cell differentiation events. Recombinant human AES protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Amino-terminal enhancer of split, AES-1, AES-2, ESP1, GRG, GRG5, TLE5, Groucho homologue

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

24.1 kDa (217aa) confirmed by MALDI-TOF

AES, Amino-terminal enhancer of split, AES-1, AES-2, ESP1, GRG, GRG5, TLE5, Groucho homologue, ATGP0954-10 µg, ATGP0954-20 µg, ATGP0954-50 µg, ATGP0954-100 µg, ATGP0954-250 µg, ATGP0954-500 µg, ATGP0954-1 mg, ATGP0954-10, ATGP0954-20, ATGP0954-50, ATGP0954-100, ATGP0954-250, ATGP0954-500, ATGP0954-1

Hou E W., et al. (1998) DNA Cell Biol. 17:911-913.; ; Yochum G S., et al. (2001) Mol Cell Biol. 21:4110-4118.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol

Signal Transduction

NP_001121

Q08117

Human

MMFPQSRHSG SSHLPQQLKF TTSDSCDRIK DEFQLLQAQY HSLKLECDKL ASEKSEMQRH YVMYYEMSYG LNIEMHKQAE IVKRLNGICA QVLPYLSQEH QQQVLGAIER AKQVTAPELN SIIRQQLQAH QLSQLQALAL PLTPLPVGLQ PPSLPAVSAG TGLLSLSALG SQAHLSKEDK NGHDGDTHQE DDGEKSD