Recombinant humaan AGA-eiwit

AGA also known as N (4) - (beta-N-acetylglucosaminyl) -L-asparaginase is involved in the catabolism of N-linked oligosaccharides of glycoproteins. It cleaves asparagine from N-acetylglucosamines as one of the final steps in the lysosomal breakdown of glycoproteins. Recombinant human AGA, fused to His-tag at N-terminus, was expressed in E. coli

N (4) - (beta-N-acetylglucosaminyl) -L-asparaginase isoform 1, AGu, ASRG, GA

E.coli

Human

His-Tag

SDS-PAGE, Denatured

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

37 kDa (346aa)

AGA, N (4) - (beta-N-acetylglucosaminyl) -L-asparaginase isoform 1, AGu, ASRG, GA, ATGP2977-10 µg, ATGP2977-20 µg, ATGP2977-50 µg, ATGP2977-100 µg, ATGP2977-250 µg, ATGP2977-500 µg, ATGP2977-1 mg, ATGP2977-10, ATGP2977-20, ATGP2977-50, ATGP2977-100, ATGP2977-250, ATGP2977-500, ATGP2977-1

Covini D., et al. (2012) Recent Pat Anticancer Drug Discov 7 (1), 4-13

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Enzymes & Proteases

NP_000018

P20933

Human

MGSSHHHHHH SSGLVPRGSH MGSSSPLPLV VNTWPFKNAT EAAWRALASG GSALDAVESG CAMCEREQCD GSVGFGGSPD ELGETTLDAM IMDGTTMDVG AVGDLRRIKN AIGVARKVLE HTTHTLLVGE SATTFAQSMG FINEDLSTTA SQALHSDWLA RNCQPNYWRN VIPDPSKYCG PYKPPGILKQ DIPIHKETED DRGHDTIGMV VIHKTGHIAA GTSTNGIKFK IHGRVGDSPI PGAGAYADDT AGAAAATGNG DILMRFLPSY QAVEYMRRGE DPTIACQKVI SRIQKHFPEF FGAVICANVT GSYGAACNKL STFTQFSFMV YNSEKNQPTE EKVDCI