Recombinant humaan AGA-eiwit

AGA, as known as N (4) - (beta-N-acetylglucosaminyl) -L-asparaginase isoform 1, belongs to the N-terminal nucleophile (Ntn) hydrolase superfamily. This protein consists of different S100 proteins and then plays various roles in regulation of protein phosphorylation, the dynamics of cytoskeleton constituents, transcription factors, enzyme activities, cell growth and differentiation, and inflammatory response. Recombinant human AGA, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

N (4) - (beta-N-acetylglucosaminyl) -L-asparaginase isoform 1, AGA, AGU, ASRG, GA

Baculovirus

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

35.7 kDa (332aa)

AGA, N (4) - (beta-N-acetylglucosaminyl) -L-asparaginase isoform 1, AGA, AGU, ASRG, GA, ATGP3440-10 µg, ATGP3440-20 µg, ATGP3440-50 µg, ATGP3440-100 µg, ATGP3440-250 µg, ATGP3440-500 µg, ATGP3440-1 mg, ATGP3440-10, ATGP3440-20, ATGP3440-50, ATGP3440-100, ATGP3440-250, ATGP3440-500, ATGP3440-1

Saarela J., et al. (2004) Biochem. J. 378:363-371.; ; Ikonen E., et al. (1992) Hum. Mutat. 1:361-365.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) containing 10% glycerol

Cell Cycle

NP_000018

P20933

Human

Ala

SSPLPLV VNTWPFKNAT EAAWRALASG GSALDAVESG CAMCEREQCD GSVGFGGSPD ELGETTLDAM IMDGTTMDVG AVGDLRRIKN AIGVARKVLE HTTHTLLVGE SATTFAQSMG FINEDLSTTA SQALHSDWLA RNCQPNYWRN VIPDPSKYCG PYKPPGILKQ DIPIHKETED DRGHDTIGMV VIHKTGHIAA GTSTNGIKFK IHGRVGDSPI PGAGAYADDT AGAAAATGNG DILMRFLPSY QAVEYMRRGE DPTIACQKVI SRIQKHFPEF FGAVICANVT GSYGAACNKL STFTQFSFMV YNSEKNQPTE EKVDCI