Recombinant humaan AgRP-eiwit

AGRP, also known as agouti-related protein also called agouti-related peptide, is a neuropeptide produced in the brain by the AGRP/NPY neuron. It is only synthesised in NPY-containing cell bodies located in the ventromedial part of the arcuate nucleus in the hypothalamus. AGRP is co-expressed with Neuropeptide Y and works by increasing appetite and decreasing metabolism and energy expenditure. It is one of the most potent and long-lasting of appetite stimulators. Recombinant human AGRP, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Agouti-related protein, Agouti-related protein, AGRT, ART, ASIP2

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

15.1 kDa (137aa) confirmed by MALDI-TOF

AgRP, Agouti-related protein, Agouti-related protein, AGRT, ART, ASIP2, ATGP3018-10 µg, ATGP3018-20 µg, ATGP3018-50 µg, ATGP3018-100 µg, ATGP3018-250 µg, ATGP3018-500 µg, ATGP3018-1 mg, ATGP3018-10, ATGP3018-20, ATGP3018-50, ATGP3018-100, ATGP3018-250, ATGP3018-500, ATGP3018-1

Xiao E, Xia-Zhang L, et al. (2003) . Endocrinology. 144 (5) :1736-41.; ; Creemers JW, Pritchard LE, et al. (2006) . Endocrinology. 147 (4) :1621-31.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 20% glycerol, 1mM DTT

Cardiovascular

NP_001129

O00253

Human

AQMGL APMEGIRRPD QALLPELPGL GLRAPLKKTT AEQAEEDLLQ EAQALAEVLD LQDREPRSSR RCVRLHESCL GQQVPCCDPC ATCYCRFFNA FCYCRKLGTA MNPCSRT