Recombinant humaan AHSP-eiwit

AHSP (Alpha-hemoglobin stabilizing protein), also known as ERAF (Erythroid associated factor), is an erythroid-specific protein that acts as a chaperone to prevent the aggregation of alpha-hemoglobin during normal erythroid cell development. It specifically protects free alpha-hemoglobin from precipitation in live cells and in solution. This protein is downregulated in transmissible spongiform encephalopathies (TSEs) . It is predicted to modulate pathological states of alpha-hemoglobin excess such as beta-thalassemia. Recombinant AHSP protein was expressed in E. coli and purified by using conventional chromatography techniques.

Alpha-hemoglobin stabilizing protein, ERAF, EDRF, Alpha-hemoglobin stabilizing protein Alpha hemoglobin stabilizing protein, Erythroid associated factor, Erythroid differentiation associated factor, Erythroid differentiation related factor

E.coli

Human

Non-Tagged

SDS-PAGE

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

11.8 kDa (102aa) confirmed by MALDI-TOF

AHSP, Alpha-hemoglobin stabilizing protein, ERAF, EDRF, Alpha-hemoglobin stabilizing protein Alpha hemoglobin stabilizing protein, Erythroid associated factor, Erythroid differentiation associated factor, Erythroid differentiation related factor, ATGP0369-10 µg, ATGP0369-20 µg, ATGP0369-50 µg, ATGP0369-100 µg, ATGP0369-250 µg, ATGP0369-500 µg, ATGP0369-1 mg, ATGP0369-10, ATGP0369-20, ATGP0369-50, ATGP0369-100, ATGP0369-250, ATGP0369-500, ATGP0369-1

Dos Santos CO., et al. (2008) J Biol Chem. 283 (40) :26956-64. ; ; Sekijima Y., et al. (2005) Cell. 121 (1) :73-85.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Heat Shock Proteins

NP_057717.1

Q9NZD4

Human

MALLKANKDL ISAGLKEFSV LLNQQVFNDP LVSEEDMVTV VEDWMNFYIN YYRQQVTGEP QERDKALQEL RQELNTLANP FLAKYRDFLK SHELPSHPPP SS