Recombinant humaan AIDA-eiwit

AIDA acts as a ventralizing factor during embryogenesis. This protein inhibits axin-mediated JNK activation by binding axin and disrupting axin homodimerization. This in turn antagonizes a Wnt/beta-catenin-independent dorsalization pathway activated by AXIN/JNK-signaling. Recombinant human AIDA protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Axin interactor dorsalization-associated, C1orf80, RP11-378J18.7

E.coli

Human

His-Tag

SDS-PAGE

0.25 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

37.4 kDa (329aa) confirmed by MALDI-TOF

AIDA, Axin interactor dorsalization-associated, Axin interactor, dorsalization-associated, C1orf80, RP11-378J18.7, ATGP1977-10 µg, ATGP1977-20 µg, ATGP1977-50 µg, ATGP1977-100 µg, ATGP1977-250 µg, ATGP1977-500 µg, ATGP1977-1 mg, ATGP1977-10, ATGP1977-20, ATGP1977-50, ATGP1977-100, ATGP1977-250, ATGP1977-500, ATGP1977-1

Rui, Y., et al. (2007) Dev. Cell 13 (2), 268-282

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.2M NaCl,1mM DTT

Development

NP_073742

Q96BJ3

Human

MGSSHHHHHH SSGLVPRGSH MGSMSEVTRS LLQRWGASFR RGADFDSWGQ LVEAIDEYQI LARHLQKEAQ AQHNNSEFTE EQKKTIGKIA TCLELRSAAL QSTQSQEEFK LEDLKKLEPI LKNILTYNKE FPFDVQPVPL RRILAPGEEE NLEFEEDEEE GGAGAGSPDS FPARVPGTLL PRLPSEPGMT LLTIRIEKIG LKDAGQCIDP YITVSVKDLN GIDLTPVQDT PVASRKEDTY VHFNVDIELQ KHVEKLTKGA AIFFEFKHYK PKKRFTSTKC FAFMEMDEIK PGPIVIELYK KPTDFKRKKL QLLTKKPLYL HLHQTLHKE