Recombinant humaan AIMP3/EEF1E1-eiwit

EEF1E1, also known as, eukaryotic translation elongation factor 1 epsilon-1, shares sequence similarity with the amino-terminal ends of the Beta and Gamma subunits of EF-1. By specifically interacting with MetRS, this protein binds to a macromolecular tRNA synthtase complex that catalyzes the ligation of specific amino acids to their cognate tRNAs. upon DNA damage, it translocates to the nucleus where it interacts with ATM and ATR, resulting in p53 activation. Recombinant human EEIF1E1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Eukaryotic translation elongation factor 1 epsilon-1, AIMP3, P18, Aminoacyl tRNA synthetase complex-interacting multifunctional protein 3, Multisynthetase complex auxiliary component p18, Elongation factor p18

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

21.9 kDa (194aa) confirmed by MALDI-TOF

EEF1E1, Eukaryotic translation elongation factor 1 epsilon-1, AIMP3, P18, ATGP1022-10 µg, ATGP1022-20 µg, ATGP1022-50 µg, ATGP1022-100 µg, ATGP1022-250 µg, ATGP1022-500 µg, ATGP1022-1 mg, ATGP1022-10, ATGP1022-20, ATGP1022-50, ATGP1022-100, ATGP1022-250, ATGP1022-500, ATGP1022-1

Quevillon S. et al. (1996) FEBS Lett. 395: 63-67.; ; Norcum MT. et al. (1991) J Biol Chem. 266:15398-405

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Cell Cycle

NP_004271

O43324

Human

MAAAAELSLL EKSLGLSKGN KYSAQGERQI PVLQTNNGPS LTGLTTIAAH LVKQANKEYL LGSTAEEKAI VQQWLEYRVT QVDGHSSKND IHTLLKDLNS YLEDKVYLTG YNFTLADILL YYGLHRFIVD LTVQEKEKYL NVSRWFCHIQ HYPGIRQHLS SVVFIKNRLY TNSH