Recombinant humaan AK4 (C22S) eiwit

Adenylate kinase (AK; adenosine triphosphate-adenosine monophosphate [ATP-AMP] phospho-transferase, EC 2. 7. 4. 3) is a ubiquitous monomeric enzyme involved energy metabolism of prokaryotic and eukaryotic cells. Five isozymes of adenylate kinase have been identified in vertebrates. AK1 is present in the cytosol of skeletal muscle, brain, and erythrocyte, while AK2 is localized in the intermembrane space of mitochondria of liver, kidney, spleen and heart. AK3, called GTP:AMP phosphotransferase, exists in the mitochondrial matrix of liver and heart. These isozymes contribute to homeostasis of the adenine nucleotide composition in the cell. Recombinant human AK3, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

AK3, AKL3L, Adenylate kinase 3 alpha like 1, Adenylate kinase 3, AK3L1, AK6, AKL3L1, FIX, RP11 6J24.4.

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

29.3 kDa (259aa) confirmed by MALDI-TOF

Adenylate kinase3 (C22S), AK3, AKL3L, Adenylate kinase 3 alpha like 1, Adenylate kinase 3, AK3L1, AK6, AKL3L1, FIX, RP11 6J24.4., ADK0801-10 µg, ADK0801-20 µg, ADK0801-50 µg, ADK0801-100 µg, ADK0801-250 µg, ADK0801-500 µg, ADK0801-1 mg, ADK0801-10, ADK0801-20, ADK0801-50, ADK0801-100, ADK0801-250, ADK0801-500, ADK0801-1

Nobumoto M., et al. (1998) J. Biol. Chem. 123 (1) :128-35; ; Noma T., et al. (2001) J. Biol. Chem. 358 (Pt 1) :225-32;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.5) containing 2mM DTT, 30% glycerol, 0.1M NaCl

Signal Transduction

NP_982289

P27144

Human

MASK LLRAVILGPP GSGKGTVSQR IAQNFGLQHL SSGHFLRENI KASTEVGEMA KQYIEKSLLV PDHVITRLMM SELENRRGQH WLLDGFPRTL GQAEALDKIC EVDLVISLNI PFETLKDRLS RRWIHPPSGR VYNLDFNPPH VHGIDDVTGE PLVQQEDDKP EAVAARLRQY KDVAKPVIEL YKSRGVLHQF SGTETNKIWP YVYTLFSNKI TPIQSKEAY