Recombinant humaan AK4-eiwit

AK4 (adenylate kinase 4) is a member of the adenylate kinase family of enzymes. This protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Inherited mutations leading to AK4 deficiencies in erythrocytes have been implicated in hemolytic anemia. Recombinant human AK4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Adenylate kinase 4, AK3, AK3L1, AK3L2, MGC166959

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

27.4 kDa (243aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)

AK4, Adenylate kinase 4, AK3, AK3L1, AK3L2, MGC166959, ATGP0880-10 µg, ATGP0880-20 µg, ATGP0880-50 µg, ATGP0880-100 µg, ATGP0880-250 µg, ATGP0880-500 µg, ATGP0880-1 mg, ATGP0880-10, ATGP0880-20, ATGP0880-50, ATGP0880-100, ATGP0880-250, ATGP0880-500, ATGP0880-1

Noma T., et al. (2001) Biochem J. 358 (1) :225-32. ; ; Dzeja PP., et al. (1998) Mol Cell Biochem. 184 (1-2) :169-82.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 2mM DTT, 0.1M NaCl.

Metabolism

NP_982289

P27144

Human

MASKLLRAVI LGPPGSGKGT VCQRIAQNFG LQHLSSGHFL RENIKASTEV GEMAKQYIEK SLLVPDHVIT RLMMSELENR RGQHWLLDGF PRTLGQAEAL DKICEVDLVI SLNIPFETLK DRLSRRWIHP PSGRVYNLDF NPPHVHGIDD VTGEPLVQQE DDKPEAVAAR LRQYKDVAKP VIELYKSRGV LHQFSGTETN KIWPYVYTLF SNKITPIQSK EAY