Recombinant humaan AKAP7-eiwit

AKAP7 (A-kinase anchor protein 7 isoform gamma isoform alpha), also known as AKAP18, belongs to the A-kinase anchoring protein (AKAP) family, a group of functionally related proteins that bind to a regulatory subunit (RII) of cAMP-dependent protein kinase A (PKA) and target the enzyme to specific subcellular compartments. AKAP 7 is expressed in brain, heart, lung, pancreas and skeletal muscle. AKAP 7 binds PKA to the plasma membrane, and permits functional coupling to the L-type calcium channel. Recombinant human AKAP7 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

A-kinase anchor protein 7 isoform gamma isoform alpha, AKAP15, AKAP18

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

11.5 kDa (105aa) confirmed by MALDI-TOF

AKAP7, A-kinase anchor protein 7 isoform gamma isoform alpha, AKAP15, AKAP18, ATGP1527-10 µg, ATGP1527-20 µg, ATGP1527-50 µg, ATGP1527-100 µg, ATGP1527-250 µg, ATGP1527-500 µg, ATGP1527-1 mg, ATGP1527-10, ATGP1527-20, ATGP1527-50, ATGP1527-100, ATGP1527-250, ATGP1527-500, ATGP1527-1

Trotter K.W., et al. (1999) J. Cell Biol. 147:1481-1492; ; Klussmann, E., et al. (2001) Kidney Int. 60: 446-449.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 50 mM NaCl

Signal Transduction

NP_004833

O43687

Human

MGQLCC FPFSRDEGKI SEKNGGEPDD AELVRLSKRL VENAVLKAVQ QYLEETQNKN KPGEGSSVKT EAADQNGNDN ENNRK