Recombinant humaan Akt1-eiwit

AKT1, also known as RAC-alpha serine/threonine-protein kinase, is catalytically inactive in serum-starved primary and immortalized fibroblasts. AKT1 and the related AKT2 are activated by platelet-derived growth factor. The activation is rapid and specific, and it is abrogated by mutations in the pleckstrin homology domain of AKT1. It was shown that the activation occurs through phosphatidylinositol 3-kinase. Survival factors can suppress apoptosis in a transcription-independent manner by activating the serine/threonine kinase AKT1, which then phosphorylates and inactivates components of the apoptotic machinery. Recombinant human AKT1, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Protein kinase B, Protein kinase B alpha, Proto-oncogene c-Akt, RAC-PK-alpha

Baculovirus

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by absorbance at 280 nm)

< 1 EU per 1ug of protein (determined by LAL method)

> 90% by SDS-PAGE

56.7 kDa (488aa)

Akt1, ATGP3118-10 µg, ATGP3118-20 µg, ATGP3118-50 µg, ATGP3118-100 µg, ATGP3118-250 µg, ATGP3118-500 µg, ATGP3118-1 mg, ATGP3118-10, ATGP3118-20, ATGP3118-50, ATGP3118-100, ATGP3118-250, ATGP3118-500, ATGP3118-1

Lindhurst MJ, et al (2011) N Engl J Med. 365 (7) :611-9.

Wang Q, et al. Tumor suppressor Pdcd4 attenuates Sin1 translation to inhibit invasion in colon carcinoma. (Oncogene. 2017) {https://pubmed.ncbi.nlm.nih.gov/28692058/}

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. Phosphate-Buffered Saline (pH 7.4) 20% glycerol

Signal Transduction

NP_001014432

P31749

Human