Recombinant humaan ALAD-eiwit

The ALAD enzyme is composed of 8 identical subunits and catalyzes the condensation of 2 molecules of delta-aminolevulinate to form porphobilinogen (a precursor of heme, cytochromes and other hemoproteins) . ALAD catalyzes the second step in the porphyrin and heme biosynthetic pathway; zinc is essential for enzymatic activity. ALAD enzymatic activity is inhibited by lead and a defect in the ALAD structural gene can cause increased sensitivity to lead poisoning and acute hepatic porphyria. Recombinant human ALAD protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Delta-aminolevulinic acid dehydratase, ALADH, PBGS

E.coli

Human

His-Tag

SDS-PAGE

1 mg/mL (determined by Bradford assay)

> 85% by SDS-PAGE

38.8 kDa (354aa) confirmed by MALDI-TOF

ALAD, Delta-aminolevulinic acid dehydratase, ALADH, PBGS, ATGP1444-10 µg, ATGP1444-20 µg, ATGP1444-50 µg, ATGP1444-100 µg, ATGP1444-250 µg, ATGP1444-500 µg, ATGP1444-1 mg, ATGP1444-10, ATGP1444-20, ATGP1444-50, ATGP1444-100, ATGP1444-250, ATGP1444-500, ATGP1444-1

Li, C.. et al. (2011) Toxicol. Lett. 203 (1), 48-53; ; van Bemmel et al. (2011) Epidemiology 22 (2), 273-278

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Enzymes & Proteases

NP_000022

P13716

Human

MQPQSV LHSGYFHPLL RAWQTATTTL NASNLIYPIF VTDVPDDIQP ITSLPGVARY GVKRLEEMLR PLVEEGLRCV LIFGVPSRVP KDERGSAADS EESPAIEAIH LLRKTFPNLL VACDVCLCPY TSHGHCGLLS ENGAFRAEES RQRLAEVALA YAKAGCQVVA PSDMMDGRVE AIKEALMAHG LGNRVSVMSY SAKFASCFYG PFRDAAKSSP AFGDRRCYQL PPGARGLALR AVDRDVREGA DMLMVKPGMP YLDIVREVKD KHPDLPLAVY HVSGEFAMLW HGAQAGAFDL KAAVLEAMTA FRRAGADIII TYYTPQLLQW LKEE