Recombinant humaan Aldehyde Dehydrogenase 3-A1/ALDH3A1-eiwit

ALDH3A1, also known as Aldehyde dehydrogenase 3 family memberA1, is involved in the detoxification of alcohol-derived acetaldehyde and in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. This protein forms a cytoplasmic homodimer that preferentially oxidizes aromatic and medium-chain (6 carbons or more) saturated and unsaturated aldehyde substrates. It is thought to promote resistance to uV and 4-hydroxy-2-nonenal-induced oxidative damage in the cornea. Recombinant ALDH3A1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 5,000pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 4-nitrobenzaldehyde by NADP per minute at pH 9.0 at 37°C.

Aldehyde dehydrogenase 3 family memberA1, ALDHIII, ALDH3, Aldehyde dehydrogenase 3 family, memberA1 AHD 4.AHD C, AHD4, AHDC, ALDH III, Aldehyde dehydrogenase 3, Aldehyde dehydrogenase 3 family member A1, Aldehyde dehydrogenase 3A1, Aldehyde dehydrogenase class 3, Aldehyde dehydrogenase dimeric NADP preferring, Aldehyde dehydrogenase family 3 member A1, Aldehyde dehydrogenase family 3 subfamily A1, Aldehyde dehydrogenase isozyme 3, Aldehyde dehydrogenase type III, ALDH 3, ALDH 3A1, ALDH3 A1, ALDH3A 1, MGC10406, Stomach aldehyde dehydrogenase, Tumor associated aldehyde dehydrogenase tumor ALDH.

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

> 95% by SDS-PAGE

Specific activity is > 5,000pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 4-nitrobenzaldehyde by NADP per minute at pH 9.0 at 37°C.

52.5 kDa (473aa)

Aldehyde Dehydrogenase3-A1, ALDH3A1, Aldehyde dehydrogenase 3 family memberA1, ALDHIII, ALDH3, Aldehyde dehydrogenase 3 family, memberA1 AHD 4.AHD C, AHD4, AHDC, ALDH III, Aldehyde dehydrogenase 3, Aldehyde dehydrogenase 3 family member A1, Aldehyde dehydrogenase 3A1, Aldehyde dehydrogenase class 3, Aldehyde dehydrogenase dimeric NADP preferring, Aldehyde dehydrogenase family 3 member A1, Aldehyde dehydrogenase family 3 subfamily A1, Aldehyde dehydrogenase isozyme 3, Aldehyde dehydrogenase type III, ALDH 3, ALDH 3A1, ALDH3 A1, ALDH3A 1, MGC10406, Stomach aldehyde dehydrogenase, Tumor associated aldehyde dehydrogenase tumor ALDH., ATGP0472-10 µg, ATGP0472-20 µg, ATGP0472-50 µg, ATGP0472-100 µg, ATGP0472-250 µg, ATGP0472-500 µg, ATGP0472-1 mg, ATGP0472-10, ATGP0472-20, ATGP0472-50, ATGP0472-100, ATGP0472-250, ATGP0472-500, ATGP0472-1

Estey T., et al. (2007) Exp Eye Res. 84 (1) :3-12; ; Bogucka M, et al. (2009) Acta Pol Pharm. 66 (5) :477-82.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol 0.1M NaCl

Redox Proteins

AAH04370.1

P30838

Human

MSKISEAVKR ARAAFSSGRT RPLQFRIQQL EALQRLIQEQ EQELVGALAA DLHKNEWNAY YEEVVYVLEE IEYMIQKLPE WAADEPVEKT PQTQQDELYI HSEPLGVVLV IGTWNYPFNL TIQPMVGAIA AGNAVVLKPS ELSENMASLL ATIIPQYLDK DLYPVINGGV PETTELLKER FDHILYTGST GVGKIIMTAA AKHLTPVTLE LGGKSPCYVD KNCDLDVACR RIAWGKFMNS GQTCVAPDYI LCDPSIQNQI VEKLKKSLKE FYGEDAKKSR DYGRIISARH FQRVMGLIEG QKVAYGGTGD AATRYIAPTI LTDVDPQSPV MQEEIFGPVL PIVCVRSLEE AIQFINQREK PLALYMFSSN DKVIKKMIAE TSSGGVAAND VIVHITLHSL PFGGVGNSGM GSYHGKKSFE TFSHRRSCLV RPLMNDEGLK VRYPPSPAKM TQH