Recombinant humaan ALDH5A1-eiwit

ALDH5A1 belongs to the aldehyde dehydrogenase family of proteins. This protein functions as a mediate the NADP+-dependent oxidation of aldehydes into acids and plays an important role in the detoxification of alcohol-derived acetaldehyde, as well as in lipid peroxidation and in the metabolism of corticosteroids, biogenic amines and neurotransmitters. ALDH5A1 is expressed in a variety of tissues, including liver, heart, lung, brain, kidney and placenta. Recombinant human ALDH5A1 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Succinate-semialdehyde dehydrogenase mitochondrial, SSADH, SSDH, Succinate-semialdehyde dehydrogenase, mitochondrial

E.coli

Human

His-Tag

SDS-PAGE

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

54.6 kDa (509aa) confirmed by MALDI-TOF

ALDH5A1, Succinate-semialdehyde dehydrogenase mitochondrial, SSADH, SSDH, Succinate-semialdehyde dehydrogenase, mitochondrial, ATGP0723-10 µg, ATGP0723-20 µg, ATGP0723-50 µg, ATGP0723-100 µg, ATGP0723-250 µg, ATGP0723-500 µg, ATGP0723-1 mg, ATGP0723-10, ATGP0723-20, ATGP0723-50, ATGP0723-100, ATGP0723-250, ATGP0723-500, ATGP0723-1

Thomasson HR., et al. (1991) Am J Hum Genet. 48 (4) :677-81. ; ; Akaboshi S., et al. (2003) Hum Mutat. 22 (6) :442-50.;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol, 1mM DTT, 0.1M NaCl, 1mM EDTA

Metabolism

NP_001071.1

P51649

Human

AGRLAGLSA ALLRTDSFVG GRWLPAAATF PVQDPASGAA LGMVADCGVR EARAAVRAAY EAFCRWREVS AKERSSLLRK WYNLMIQNKD DLARIITAES GKPLKEAHGE ILYSAFFLEW FSEEARRVYG DIIHTPAKDR RALVLKQPIG VAAVITPWNF PSAMITRKVG AALAAGCTVV VKPAEDTPFS ALALAELASQ AGIPSGVYNV IPCSRKNAKE VGEAICTDPL VSKISFTGST TTGKILLHHA ANSVKRVSME LGGLAPFIVF DSANVDQAVA GAMASKFRNT GQTCVCSNQF LVQRGIHDAF VKAFAEAMKK NLRVGNGFEE GTTQGPLINE KAVEKVEKQV NDAVSKGATV VTGGKRHQLG KNFFEPTLLC NVTQDMLCTH EETFGPLAPV IKFDTEEEAI AIANAADVGL AGYFYSQDPA QIWRVAEQLE VGMVGVNEGL ISSVECPFGG VKQSGLGREG SKYGIDEYLE LKYVCYGGL