Recombinant humaan Aldo-keto Reductase 1C1/AKR1C1-eiwit

AKR1C1 is member of the aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. These enzymes catalyze the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. The enzymes display overlapping but distinct substrate specificity. This enzyme catalyzes the reduction of progesterone to the inactive form 20-alpha-hydroxy-progesterone. Recombinant AKR1C1 protein was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 500 pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.

Aldo-keto reductase family 1 member C1, DDH1, DDH, MBAB, DD1, HAKRC, Dihydrodiol dehydrogenase 1, 20-alpha (3-alpha) -hydroxysteroid dehydrogenase, High-affinity hepatic bile acid-binding protein (HBAB)

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

0.5 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is > 500pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.

38.9 kDa (343aa) confirmed by MALDI-TOF

Aldo-keto Reductase1C1, AKR1C1, Aldo-keto reductase family 1 member C1, 20-alpha-HSD, DD1/DD2, HBAB, DDH, DDH1, Aldo-keto reductase family 1, member C1 20 alpha (3 alpha) hydroxysteroid dehydrogenase, 20 alpha hydroxysteroid dehydrogenase, 2ALPHAHSD, AK1C1, MBAB, Aldo keto reductase family 1 member C1, C9, Chlordecone reductase homolog, Chlordecone reductase homolog HAKRC, DD1, Dihydrodiol dehydrogenase 1, Dihydrodiol dehydrogenase 1/2, H37, HAKRC, Hepatic dihydrodiol dehydrogenase, High affinity hepatic bile acid-binding protein, Trans-1,2 dihydrobenzene 1,2 diol dehydrogenase, Type II 3 alpha hydroxysteroid dehydrogenase, ATGP0496-10 µg, ATGP0496-20 µg, ATGP0496-50 µg, ATGP0496-100 µg, ATGP0496-250 µg, ATGP0496-500 µg, ATGP0496-1 mg, ATGP0496-10, ATGP0496-20, ATGP0496-50, ATGP0496-100, ATGP0496-250, ATGP0496-500, ATGP0496-1

Zhang Y., et al. (2000) J. Mol. Endocrinol. 25:221-228; ; Zhang Y., et al. (2009) Mol Cell Endicrinol. 298 (1-2) :76-83;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 20% glycerol

Redox Proteins

NP_001344

Q04828

Human

MGSSHHHHHH SSGLVPRGSH MDSKYQCVKL NDGHFMPVLG FGTYAPAEVP KSKALEATKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCNSH RPELVRPALE RSLKNLQLDY VDLYLIHFPV SVKPGEEVIP KDENGKILFD TVDLCATWEA VEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNQR KLLDFCKSKD IVLVAYSALG SHREEPWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTS EEMKAIDGLN RNVRYLTLDI FAGPPNYPFS DEY