Recombinant humaan Aldo-keto Reductase 1C3/AKR1C3-eiwit

AKR1C3, also known as PGFS, is a member of the aldo-keto reductase superfamily which catalyzes the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. This enzyme catalyzes the reduction of prostaglandin (PG) D2, PGH2 and phenanthrenequinone (PQ), and the oxidation of 9 alpha, 11 beta-PGF2 to PGD2. It may play an important role in the pathogenesis of allergic diseases such as asthma, and may also have a role in controlling cell growth and differentiation. Recombinant human AKR1C3 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specific activity is > 1,000 pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.

Aldo-keto reductase family 1 member C3, DD3, DDX, HA1753, HAKRB, HAKRe, hluPGFS, HSD17B5, Aldo-keto reductase family 1, member C3 17 beta HSD 5 (HSD17B5), AKR1 C3, Chlordecone reductase, 17 beta hydroxysteroid dehydrogenase type 5, DDH1, PGFS, Prostaglandin F synthase

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

< 1 EU per 1ug of protein (determined by LAL method)

> 95% by SDS-PAGE

Specific activity is > 1,000pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.

39 kDa (343aa) confirmed by MALDI-TOF

Aldo-keto Reductase1C3, AKR1C3, Aldo-keto reductase family 1 member C3, Aldo-keto reductase family 1, member C3, DD3, DDX, HA1753, HAKRB, HAKRe, hluPGFS, HSD17B5, KIAA0119, Aldo-keto reductase family 1, member C3 17 beta HSD 5, AKR1 C3, Chlordecone reductase, 17 beta hydroxysteroid dehydrogenase type 5, Aldo keto reductase family 1 member C3, DDH1, HAKRe, HSD17B5, PGFS, Prostaglandin F synthase, ATGP0270-10 µg, ATGP0270-20 µg, ATGP0270-50 µg, ATGP0270-100 µg, ATGP0270-250 µg, ATGP0270-500 µg, ATGP0270-1 mg, ATGP0270-10, ATGP0270-20, ATGP0270-50, ATGP0270-100, ATGP0270-250, ATGP0270-500, ATGP0270-1

Davies N., et al. (2009) Cancer Res. 69 (11) :4769-75; ; Kabututu Z., et al. (2009) J Biochem. 145 (2) :161-8;

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 10% glycerol

Redox Proteins

NP_003730.4

P42330

Human

MDSKHQCVKL NDGHFMPVLG FGTYAPPEVP RSKALEVTKL AIEAGFRHID SAHLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWSTFH RPELVRPALE NSLKKAQLDY VDLYLIHSPM SLKPGEELSP TDENGKVIFD IVDLCTTWEA MEKCKDAGLA KSIGVSNFNR RQLEMILNKP GLKYKPVCNQ VECHPYFNRS KLLDFCKSKD IVLVAYSALG SQRDKRWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIRQN VQVFEFQLTA EDMKAIDGLD RNLHYFNSDS FASHPNYPYS DEY