Recombinant humaan Aldo-keto Reductase 1C4/AKR1C4-eiwit

AKR1C4 belongs to aldo/keto reductase superfamily, which consists of more than 40 known enzymes and proteins. It catalyzes the conversion of aldehydes and ketones to their corresponding alcohols by utilizing NADH and/or NADPH as cofactors. This enzyme catalyzes the bioreduction of chlordecone, a toxic organochlorine pesticide, to chlordecone alcohol in liver. Recombinant human AKR1C4 protein, fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography.

Specific activity is > 700 pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0 pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.

E.coli

Human

His-Tag

SDS-PAGE, Enzyme Activity

1 mg/mL (determined by Bradford assay)

> 90% by SDS-PAGE

Specific activity is > 700pmol/min/ug, and is defined as the amount of enzyme that catalyze the oxidation of 1.0pmole 1-Acenaphthenol in the presence of NADP per minute at pH 8.8 at 25C.

39.2 kDa (343aa) confirmed by MALDI-TOF

Type I, ST2A3, ST2A1, ST2, member C4, hSTa, HST, HAKRA, dihydrodiol dehydrogenase 4, DHEA-ST, DHEAS, DD4, chlordecone reductase, CHDR, CDR, C11, Aldo-keto Reductase1C4, Aldo-keto reductase family 1 member C4, aldo-keto reductase family 1, AKR1C4, 3-alpha-HSD, 3-alpha hydroxysteroid dehydrogenase type I, 3-alpha hydroxysteroid dehydrogenase, ATGP1106-10 µg, ATGP1106-20 µg, ATGP1106-50 µg, ATGP1106-100 µg, ATGP1106-250 µg, ATGP1106-500 µg, ATGP1106-1 mg, ATGP1106-010, ATGP1106-020, ATGP1106-050, ATGP1106-100, ATGP1106-250, ATGP1106-500, ATGP1106-01M

Khanna M. et al. (1995) Genomics. 25:588-90.; ; Stayrook KR. et al. (2008) Mol Pharmacol. 73:607-612.

Can be stored at 2°C to 8°C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles.

Liquid in. 20 mM Tris-HCl buffer (pH 8.0) containing 20% glycerol, 0.1M NaCl,1mM DTT

Enzymes & Proteases

NP_001809

P17516

Human

MGSSHHHHHH SSGLVPRGSH MDPKYQRVEL NDGHFMPVLG FGTYAPPEVP RNRAVEVTKL AIEAGFRHID SAYLYNNEEQ VGLAIRSKIA DGSVKREDIF YTSKLWCTFF QPQMVQPALE SSLKKLQLDY VDLYLLHFPM ALKPGETPLP KDENGKVIFD TVDLSATWEV MEKCKDAGLA KSIGVSNFNC RQLEMILNKP GLKYKPVCNQ VECHPYLNQS KLLDFCKSKD IVLVAHSALG TQRHKLWVDP NSPVLLEDPV LCALAKKHKR TPALIALRYQ LQRGVVVLAK SYNEQRIREN IQVFEFQLTS EDMKVLDGLN RNYRYVVMDF LMDHPDYPFS DEY